Detection of residual B precursor lymphoblastic leukemia by uniform gating flow cytometry

Ester Mejstříková; Eva Froňková; Tomáš Kalina; Marek Omelka; Drago Batinić; Klara Dubravčić; Klára Pospíšilová; Martina Vášková; Drorit Luria; Suk Hang Cheng; Margaret Ng; Yonna Leung; Janos Kappelmayer; Flora Kiss; Shai Izraeli; Batia Stark; Martin Schrappe; Jan Trka; Jan Starý; Ondřej Hrušák

doi:10.1002/pbc.22261

Detection of residual B precursor lymphoblastic leukemia by uniform gating flow cytometry

Ester Mejstříková, Eva Froňková, Tomáš Kalina, Marek Omelka, Drago Batinić, Klara Dubravčić, Klára Pospíšilová, Martina Vášková, Drorit Luria, Suk Hang Cheng, Margaret Ng, Yonna Leung, Janos Kappelmayer, Flora Kiss, Shai Izraeli, Batia Stark, Martin Schrappe, Jan Trka, Jan Starý, Ondřej Hrušák^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Background. Residual disease (RD) is an important prognostic factor in acute lymphoblastic leukemia (ALL). Flow cytometry (FC)-based RD detection is easy to perform, but interpretation requires expert analysis due to individual differences among patients. Procedure. We focused at the design of standardized and reproducible RD monitoring in ALL. RD was investigated by a uniform gating strategy, which was designed internationally and tested in one center by Ig/TCR rearrangements. Results. For each gate, positivity cutoff value was assigned using quantification of non-leukemic background. Comparing to Ig/TCR at 0.1% level, 80 of 103 specimens were correctly diagnosed by FC. The predictive value of FC RD at day 15 was then analyzed. In B lineage ALL, day 15 FC significantly correlated with Ig/TCR results at day 33 and/or week 12 (P<0.01). No significant correlation was found in T lineage ALL. Conclusions. Thus, FC with preset uniform gating at day 15 predicts PCR-detectable MRD in B precursor ALL. Presented data may be used to define new polychromatic cytometric diagnostics of MRD including semiautomatic assessment.

Original language	English
Pages (from-to)	62-70
Number of pages	9
Journal	Pediatric Blood and Cancer
Volume	54
Issue number	1
DOIs	https://doi.org/10.1002/pbc.22261
State	Published - Jan 2010
Externally published	Yes

Keywords

Acute lymphoblastic leukemia
Flow cytometry
Minimal residual disease
Non-malignant background

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10.1002/pbc.22261

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Mejstříková, E., Froňková, E., Kalina, T., Omelka, M., Batinić, D., Dubravčić, K., Pospíšilová, K., Vášková, M., Luria, D., Cheng, S. H., Ng, M., Leung, Y., Kappelmayer, J., Kiss, F., Izraeli, S., Stark, B., Schrappe, M., Trka, J., Starý, J., & Hrušák, O. (2010). Detection of residual B precursor lymphoblastic leukemia by uniform gating flow cytometry. Pediatric Blood and Cancer, 54(1), 62-70. https://doi.org/10.1002/pbc.22261

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title = "Detection of residual B precursor lymphoblastic leukemia by uniform gating flow cytometry",

abstract = "Background. Residual disease (RD) is an important prognostic factor in acute lymphoblastic leukemia (ALL). Flow cytometry (FC)-based RD detection is easy to perform, but interpretation requires expert analysis due to individual differences among patients. Procedure. We focused at the design of standardized and reproducible RD monitoring in ALL. RD was investigated by a uniform gating strategy, which was designed internationally and tested in one center by Ig/TCR rearrangements. Results. For each gate, positivity cutoff value was assigned using quantification of non-leukemic background. Comparing to Ig/TCR at 0.1% level, 80 of 103 specimens were correctly diagnosed by FC. The predictive value of FC RD at day 15 was then analyzed. In B lineage ALL, day 15 FC significantly correlated with Ig/TCR results at day 33 and/or week 12 (P<0.01). No significant correlation was found in T lineage ALL. Conclusions. Thus, FC with preset uniform gating at day 15 predicts PCR-detectable MRD in B precursor ALL. Presented data may be used to define new polychromatic cytometric diagnostics of MRD including semiautomatic assessment.",

keywords = "Acute lymphoblastic leukemia, Flow cytometry, Minimal residual disease, Non-malignant background",

author = "Ester Mejst{\v r}{\'i}kov{\'a} and Eva Fro{\v n}kov{\'a} and Tom{\'a}{\v s} Kalina and Marek Omelka and Drago Batini{\'c} and Klara Dubrav{\v c}i{\'c} and Kl{\'a}ra Posp{\'i}{\v s}ilov{\'a} and Martina V{\'a}{\v s}kov{\'a} and Drorit Luria and Cheng, {Suk Hang} and Margaret Ng and Yonna Leung and Janos Kappelmayer and Flora Kiss and Shai Izraeli and Batia Stark and Martin Schrappe and Jan Trka and Jan Star{\'y} and Ond{\v r}ej Hru{\v s}{\'a}k",

year = "2010",

month = jan,

doi = "10.1002/pbc.22261",

language = "אנגלית",

volume = "54",

pages = "62--70",

journal = "Pediatric Blood and Cancer",

issn = "1545-5009",

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Mejstříková, E, Froňková, E, Kalina, T, Omelka, M, Batinić, D, Dubravčić, K, Pospíšilová, K, Vášková, M, Luria, D, Cheng, SH, Ng, M, Leung, Y, Kappelmayer, J, Kiss, F, Izraeli, S , Stark, B, Schrappe, M, Trka, J, Starý, J & Hrušák, O 2010, 'Detection of residual B precursor lymphoblastic leukemia by uniform gating flow cytometry', Pediatric Blood and Cancer, vol. 54, no. 1, pp. 62-70. https://doi.org/10.1002/pbc.22261

TY - JOUR

T1 - Detection of residual B precursor lymphoblastic leukemia by uniform gating flow cytometry

AU - Mejstříková, Ester

AU - Froňková, Eva

AU - Kalina, Tomáš

AU - Omelka, Marek

AU - Batinić, Drago

AU - Dubravčić, Klara

AU - Pospíšilová, Klára

AU - Vášková, Martina

AU - Luria, Drorit

AU - Cheng, Suk Hang

AU - Ng, Margaret

AU - Leung, Yonna

AU - Kappelmayer, Janos

AU - Kiss, Flora

AU - Izraeli, Shai

AU - Stark, Batia

AU - Schrappe, Martin

AU - Trka, Jan

AU - Starý, Jan

AU - Hrušák, Ondřej

PY - 2010/1

Y1 - 2010/1

N2 - Background. Residual disease (RD) is an important prognostic factor in acute lymphoblastic leukemia (ALL). Flow cytometry (FC)-based RD detection is easy to perform, but interpretation requires expert analysis due to individual differences among patients. Procedure. We focused at the design of standardized and reproducible RD monitoring in ALL. RD was investigated by a uniform gating strategy, which was designed internationally and tested in one center by Ig/TCR rearrangements. Results. For each gate, positivity cutoff value was assigned using quantification of non-leukemic background. Comparing to Ig/TCR at 0.1% level, 80 of 103 specimens were correctly diagnosed by FC. The predictive value of FC RD at day 15 was then analyzed. In B lineage ALL, day 15 FC significantly correlated with Ig/TCR results at day 33 and/or week 12 (P<0.01). No significant correlation was found in T lineage ALL. Conclusions. Thus, FC with preset uniform gating at day 15 predicts PCR-detectable MRD in B precursor ALL. Presented data may be used to define new polychromatic cytometric diagnostics of MRD including semiautomatic assessment.

AB - Background. Residual disease (RD) is an important prognostic factor in acute lymphoblastic leukemia (ALL). Flow cytometry (FC)-based RD detection is easy to perform, but interpretation requires expert analysis due to individual differences among patients. Procedure. We focused at the design of standardized and reproducible RD monitoring in ALL. RD was investigated by a uniform gating strategy, which was designed internationally and tested in one center by Ig/TCR rearrangements. Results. For each gate, positivity cutoff value was assigned using quantification of non-leukemic background. Comparing to Ig/TCR at 0.1% level, 80 of 103 specimens were correctly diagnosed by FC. The predictive value of FC RD at day 15 was then analyzed. In B lineage ALL, day 15 FC significantly correlated with Ig/TCR results at day 33 and/or week 12 (P<0.01). No significant correlation was found in T lineage ALL. Conclusions. Thus, FC with preset uniform gating at day 15 predicts PCR-detectable MRD in B precursor ALL. Presented data may be used to define new polychromatic cytometric diagnostics of MRD including semiautomatic assessment.

KW - Acute lymphoblastic leukemia

KW - Flow cytometry

KW - Minimal residual disease

KW - Non-malignant background

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U2 - 10.1002/pbc.22261

DO - 10.1002/pbc.22261

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AN - SCOPUS:73349090614

SN - 1545-5009

VL - 54

SP - 62

EP - 70

JO - Pediatric Blood and Cancer

JF - Pediatric Blood and Cancer

IS - 1

ER -

Detection of residual B precursor lymphoblastic leukemia by uniform gating flow cytometry

Abstract

Keywords

UN SDGs

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