Detection of peptide-based nanoparticles in blood plasma by ELISA

Gerard H. Bode, Karin E. Pickl, Maria Sanchez-Purrà, Berta Albaiges, Salvador Borrós, Andy J.G. Pötgens, Christoph Schmitz, Frank M. Sinner, Mario Losen, Harry W.M. Steinbusch, Hans Georg Frank, Pilar Martinez-Martinez, Kurt Wagemann, Harm Anton Klok, Ronald E. Unger, Thomas Pieber, Attilio Cesàro, Johan Engbersen, Bengt Kasemo, Martin MoellerRafi Korenstein, Christian Grandfils, Andreas Bernkop-Schnuerch, Costas Kiparissides, Stanislaw Slomkowski, Peter Venturini, Constantinos M. Paleos, Barbara Podobnik, Paul Borm, Ewoud C.A. Van Winden, Juergen Groll, Birgit Zassler, Gregory Gregoriadis, Peter Kresten Nielsen, Abdelatif Elouahabi

Research output: Contribution to journalArticlepeer-review

Abstract

Aims: The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma. Materials & Methods: A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology. Results: The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL. We were able to accurately measure peptides bound to pentafluorophenyl meth-acrylate nanoparticles in blood plasma of rats, and similar results were obtained by LC/MS. Conclusions: We detected FITC-labeled peptides on pentafluorophenyl methacrylate nanoparticles after injection in vivo. This method can be extended to detect nanoparticles with different chemical compositions.

Original languageEnglish
Article numbere0126136
JournalPLoS ONE
Volume10
Issue number5
DOIs
StatePublished - 21 May 2015

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