Detection of mRNAs containing regulatory peptide coding sequences using synthetic oligodeoxynucleotides

Illana Gozes*, Mordechai Bodner, Yael Shani, Mati Fridkin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

To understand the regulation of the production of peptide hormones, it is vital to elucidate their biosynthetic pathways. We chose to study a major regulatory peptide, vasoactive intestinal peptide (VIP), a peptide possessing both neurotransmitter and neurohormone actions. To identify the specific peptide mRNA we are using, as hybridization probes, radiolabeled synthetic oligodcoxynucleotides with sequence complementary to the predicted peptide mRNA sequence. Employing this approach, we identified and partially purified a ∼ 1600‐base long mRNA containing VIP related sequences which can be translated in vitro into VIP‐immunoreactive polypeptides. Such mRNA was detected in normal VIP producing tissue (rat brain), as well as in a tumor producing VIP (human buccal tumor). This mRNA differs in size from a known VIP‐mRNA identified in human neuro‐blastoma cells, suggesting the possibility of different VIP‐mRNAs in different cell types.

Original languageEnglish
Pages (from-to)147-156
Number of pages10
JournalJournal of Cellular Biochemistry
Volume26
Issue number3
DOIs
StatePublished - 1984
Externally publishedYes

Keywords

  • VIP
  • mRNAs
  • oligodeoxynucleotides

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