Detection of fluorescent-labeled probes at sub-picomolar concentrations by magnetic modulation

Amos Danielli; Ady Arie; Noga Porat; Marcelo Ehrlich

doi:10.1364/OE.16.019253

Detection of fluorescent-labeled probes at sub-picomolar concentrations by magnetic modulation

Amos Danielli^*, Ady Arie, Noga Porat, Marcelo Ehrlich

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

A sensitive and rapid method for detecting fluorescent dyes at low concentrations in homogenous solution is experimentally demonstrated. Fluorescent-labeled DNA probes are detected by attaching magnetic beads and applying alternating magnetic field gradient. This condenses the fluorescent probes into a small detection volume and eliminates the scattering noise from solution by synchronous detection. For DNA probes concentration of 1·10^-13 M the detection signal was 3.3 times higher than the noise, thereby implying detection sensitivity of 3·10^-14 M.

Original language	English
Pages (from-to)	19253-19259
Number of pages	7
Journal	Optics Express
Volume	16
Issue number	23
DOIs	https://doi.org/10.1364/OE.16.019253
State	Published - 10 Nov 2008

Access to Document

10.1364/OE.16.019253

Cite this

@article{beff91bf8afe47099fdc0855bc32c0a7,

title = "Detection of fluorescent-labeled probes at sub-picomolar concentrations by magnetic modulation",

abstract = "A sensitive and rapid method for detecting fluorescent dyes at low concentrations in homogenous solution is experimentally demonstrated. Fluorescent-labeled DNA probes are detected by attaching magnetic beads and applying alternating magnetic field gradient. This condenses the fluorescent probes into a small detection volume and eliminates the scattering noise from solution by synchronous detection. For DNA probes concentration of 1·10-13 M the detection signal was 3.3 times higher than the noise, thereby implying detection sensitivity of 3·10-14 M.",

author = "Amos Danielli and Ady Arie and Noga Porat and Marcelo Ehrlich",

year = "2008",

month = nov,

day = "10",

doi = "10.1364/OE.16.019253",

language = "אנגלית",

volume = "16",

pages = "19253--19259",

journal = "Optics Express",

issn = "1094-4087",

publisher = "Optica Publishing Group",

number = "23",

}

TY - JOUR

T1 - Detection of fluorescent-labeled probes at sub-picomolar concentrations by magnetic modulation

AU - Danielli, Amos

AU - Arie, Ady

AU - Porat, Noga

AU - Ehrlich, Marcelo

PY - 2008/11/10

Y1 - 2008/11/10

N2 - A sensitive and rapid method for detecting fluorescent dyes at low concentrations in homogenous solution is experimentally demonstrated. Fluorescent-labeled DNA probes are detected by attaching magnetic beads and applying alternating magnetic field gradient. This condenses the fluorescent probes into a small detection volume and eliminates the scattering noise from solution by synchronous detection. For DNA probes concentration of 1·10-13 M the detection signal was 3.3 times higher than the noise, thereby implying detection sensitivity of 3·10-14 M.

AB - A sensitive and rapid method for detecting fluorescent dyes at low concentrations in homogenous solution is experimentally demonstrated. Fluorescent-labeled DNA probes are detected by attaching magnetic beads and applying alternating magnetic field gradient. This condenses the fluorescent probes into a small detection volume and eliminates the scattering noise from solution by synchronous detection. For DNA probes concentration of 1·10-13 M the detection signal was 3.3 times higher than the noise, thereby implying detection sensitivity of 3·10-14 M.

UR - http://www.scopus.com/inward/record.url?scp=56149122905&partnerID=8YFLogxK

U2 - 10.1364/OE.16.019253

DO - 10.1364/OE.16.019253

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:56149122905

SN - 1094-4087

VL - 16

SP - 19253

EP - 19259

JO - Optics Express

JF - Optics Express

IS - 23

ER -

Detection of fluorescent-labeled probes at sub-picomolar concentrations by magnetic modulation

Abstract

Access to Document

Other files and links

Fingerprint

Cite this