Detection of fluorescent-labeled probes at sub-picomolar concentrations by magnetic modulation

Amos Danielli*, Ady Arie, Noga Porat, Marcelo Ehrlich

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A sensitive and rapid method for detecting fluorescent dyes at low concentrations in homogenous solution is experimentally demonstrated. Fluorescent-labeled DNA probes are detected by attaching magnetic beads and applying alternating magnetic field gradient. This condenses the fluorescent probes into a small detection volume and eliminates the scattering noise from solution by synchronous detection. For DNA probes concentration of 1·10-13 M the detection signal was 3.3 times higher than the noise, thereby implying detection sensitivity of 3·10-14 M.

Original languageEnglish
Pages (from-to)19253-19259
Number of pages7
JournalOptics Express
Volume16
Issue number23
DOIs
StatePublished - 10 Nov 2008

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