TY - JOUR
T1 - Detection of fetal HLA‐DQα sequences in maternal blood
T2 - A gender‐independent technique of fetal cell identification
AU - Geifman‐Holtzman, Ossie
AU - Holtzman, Eliezer J.
AU - Vadnais, Theresa J.
AU - Phillips, Vincent E.
AU - Capeless, Eleanor L.
AU - Bianchi, Diana W.
PY - 1995/3
Y1 - 1995/3
N2 - The objective of this study was to detect fetal HLA‐DQα gene sequences in maternal blood. HLA‐DQα genotypes of 70 pregnant women and their partners were determined for type A1. We specifically sought couples where the father, but not the mother, had genotype A1. In 12 women, maternal blood samples were flow‐sorted. Candidate fetal cells were isolated and amplified by using PCR primers specific for a paternal HLA‐DQα A1 allele. Fetal HLA‐DQα A1 genotype was predicted from sorted cells; amniocytes or cheek swabs were used for confirmation. Six of twelve sorted samples had amplification products indicating the presence of the HLA‐DQα A1 allele; 6/12 did not. Prediction of the fetal genotype was 100 per cent correct, as determined by subsequent amplification of amniocytes or cheek swabs. We conclude that paternally inherited uniquely fetal HLA‐DQα gene sequences can be identified in maternal blood. This system permits the identification of fetal cells independent of fetal gender, and has the potential for non‐invasive prenatal diagnosis of paternally inherited conditions.
AB - The objective of this study was to detect fetal HLA‐DQα gene sequences in maternal blood. HLA‐DQα genotypes of 70 pregnant women and their partners were determined for type A1. We specifically sought couples where the father, but not the mother, had genotype A1. In 12 women, maternal blood samples were flow‐sorted. Candidate fetal cells were isolated and amplified by using PCR primers specific for a paternal HLA‐DQα A1 allele. Fetal HLA‐DQα A1 genotype was predicted from sorted cells; amniocytes or cheek swabs were used for confirmation. Six of twelve sorted samples had amplification products indicating the presence of the HLA‐DQα A1 allele; 6/12 did not. Prediction of the fetal genotype was 100 per cent correct, as determined by subsequent amplification of amniocytes or cheek swabs. We conclude that paternally inherited uniquely fetal HLA‐DQα gene sequences can be identified in maternal blood. This system permits the identification of fetal cells independent of fetal gender, and has the potential for non‐invasive prenatal diagnosis of paternally inherited conditions.
KW - HLA‐DQα
KW - fetal cells
KW - flow cytometry
KW - polymerase chain reaction
KW - prenatal diagnosis
UR - http://www.scopus.com/inward/record.url?scp=0028987678&partnerID=8YFLogxK
U2 - 10.1002/pd.1970150309
DO - 10.1002/pd.1970150309
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C2 - 7784384
AN - SCOPUS:0028987678
SN - 0197-3851
VL - 15
SP - 261
EP - 268
JO - Prenatal Diagnosis
JF - Prenatal Diagnosis
IS - 3
ER -