Detection of conformational changes in rat kidney gamma-glutamyl transpeptidase by an antibody against a synthetic peptide belonging to part of the reactive centre of the enzyme

G. A. Glass, E. Bluvshtein, Y. Gur, S. Sfez, V. Simovich, A. A. Stark*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Polyclonal antibodies were produced against rat kidney gamma-glutamyl transpeptidase (GGT) and against a synthetic peptide corresponding to residues 512-534 in rat GGT. The anti-peptide antibody bound denatured GGT, acivicin-treated GGT and GGT adsorbed to microtiter plates, but not GGT in solution, and did not inhibit GGT. The antibody against native GGT inhibited its activity in solution and did not bind efficiently adsorbed GGT in direct ELISA. It was active in direct and competition ELISA using kidney brush border membranes as the adsorbed antigen. The results indicate that these antibodies recognize conformational rather than sequence epitopes in GGT, and that marked changes occur in the conformation of GGT upon its adsorption to plates or its reaction with acivicin.

Original languageEnglish
Pages (from-to)505-513
Number of pages9
JournalBiochemistry and Molecular Biology International
Volume33
Issue number3
StatePublished - 1994

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