Detection of breast cancer cells in ductal lavage fluid by methylation-specific PCR

Ella Evron; William C. Dooley; Christopher B. Umbricht; Dorothy Rosenthal; Nicoletta Sacchi; Edward Gabrielson; Angela B. Soito; David T. Hung; Britt Marie Ljung; Nancy E. Davidson; Saraswati Sukumar

doi:10.1016/S0140-6736(00)04501-3

Detection of breast cancer cells in ductal lavage fluid by methylation-specific PCR

Ella Evron, William C. Dooley, Christopher B. Umbricht, Dorothy Rosenthal, Nicoletta Sacchi, Edward Gabrielson, Angela B. Soito, David T. Hung, Britt Marie Ljung, Nancy E. Davidson, Saraswati Sukumar^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

330 Scopus citations

Abstract

If detected early, breast cancer is curable. We tested cells collected from the breast ducts by methylation-specific PCR (MSP). Methylated alleles of Cyclin D2, RAR-β, and Twist genes were frequently detected in fluid from mammary ducts containing endoscopically visualised carcinomas (17 cases of 20), and ductal carcinoma in situ (two of seven), but rarely in ductal lavage fluid from healthy ducts (five of 45). Two of the women with healthy mammograms whose ductal lavage fluid contained methylated markers and cytologically abnormal cells were subsequently diagnosed with breast cancer. Carrying out MSP in these fluid samples may provide a sensitive and powerful addition to mammographic screening for early detection of breast cancer.

Original language	English
Pages (from-to)	1335-1336
Number of pages	2
Journal	The Lancet
Volume	357
Issue number	9265
DOIs	https://doi.org/10.1016/S0140-6736(00)04501-3
State	Published - 28 Apr 2001
Externally published	Yes

Funding

Funders	Funder number
Arthur and Rochelle Belfer Tissue Bank
Susan G Komen Foundation	BCTR 2000 577
National Institutes of Health
National Cancer Institute	P50CA088843
American Breast Cancer Foundation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0140-6736(00)04501-3

Cite this

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title = "Detection of breast cancer cells in ductal lavage fluid by methylation-specific PCR",

abstract = "If detected early, breast cancer is curable. We tested cells collected from the breast ducts by methylation-specific PCR (MSP). Methylated alleles of Cyclin D2, RAR-β, and Twist genes were frequently detected in fluid from mammary ducts containing endoscopically visualised carcinomas (17 cases of 20), and ductal carcinoma in situ (two of seven), but rarely in ductal lavage fluid from healthy ducts (five of 45). Two of the women with healthy mammograms whose ductal lavage fluid contained methylated markers and cytologically abnormal cells were subsequently diagnosed with breast cancer. Carrying out MSP in these fluid samples may provide a sensitive and powerful addition to mammographic screening for early detection of breast cancer.",

author = "Ella Evron and Dooley, {William C.} and Umbricht, {Christopher B.} and Dorothy Rosenthal and Nicoletta Sacchi and Edward Gabrielson and Soito, {Angela B.} and Hung, {David T.} and Ljung, {Britt Marie} and Davidson, {Nancy E.} and Saraswati Sukumar",

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AU - Evron, Ella

AU - Dooley, William C.

AU - Umbricht, Christopher B.

AU - Rosenthal, Dorothy

AU - Sacchi, Nicoletta

AU - Gabrielson, Edward

AU - Soito, Angela B.

AU - Hung, David T.

AU - Ljung, Britt Marie

AU - Davidson, Nancy E.

AU - Sukumar, Saraswati

PY - 2001/4/28

Y1 - 2001/4/28

N2 - If detected early, breast cancer is curable. We tested cells collected from the breast ducts by methylation-specific PCR (MSP). Methylated alleles of Cyclin D2, RAR-β, and Twist genes were frequently detected in fluid from mammary ducts containing endoscopically visualised carcinomas (17 cases of 20), and ductal carcinoma in situ (two of seven), but rarely in ductal lavage fluid from healthy ducts (five of 45). Two of the women with healthy mammograms whose ductal lavage fluid contained methylated markers and cytologically abnormal cells were subsequently diagnosed with breast cancer. Carrying out MSP in these fluid samples may provide a sensitive and powerful addition to mammographic screening for early detection of breast cancer.

AB - If detected early, breast cancer is curable. We tested cells collected from the breast ducts by methylation-specific PCR (MSP). Methylated alleles of Cyclin D2, RAR-β, and Twist genes were frequently detected in fluid from mammary ducts containing endoscopically visualised carcinomas (17 cases of 20), and ductal carcinoma in situ (two of seven), but rarely in ductal lavage fluid from healthy ducts (five of 45). Two of the women with healthy mammograms whose ductal lavage fluid contained methylated markers and cytologically abnormal cells were subsequently diagnosed with breast cancer. Carrying out MSP in these fluid samples may provide a sensitive and powerful addition to mammographic screening for early detection of breast cancer.

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Detection of breast cancer cells in ductal lavage fluid by methylation-specific PCR

Abstract

Funding

UN SDGs

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