Detection of bcl rearrangements in B-CLL by fluorescence in situ hybridization

A. Amiel, M. Lishner, A. Lalkin, E. Gaber, Y. Manor, M. Fejgin, S. Yarkoni, M. Ravid

Research output: Contribution to journalArticlepeer-review

Abstract

Data concerning oncogene activation in CLL are very limited. When studied by Southern blot, rearrangements of bcl-1, bcl-2, and bcl-3 have been only infrequently reported. We evaluated the role of fluorescence in situ hybridization (FISH) in the detection of gene rearrangements in two CLL patients. We used multiple DNA probes, including those of chromosome 12, immunoglobulin heavy and light chains, and the oncogenes bcl-1, bcl-2, and bcl-3. Additionally, routine cytogenetic study was performed. In one patient, trisomy 12 and bcl-2 translocation were demonstrated by both methods, while trisomy 12 and bcl-1 translocation were seen in the second patient, who had a normal karyotype. Larger studies should evaluate the role of FISH in the detection of oncogene involvement in CLL and compare it with other molecular methods.

Original languageEnglish
Pages (from-to)165-168
Number of pages4
JournalCancer Genetics and Cytogenetics
Volume73
Issue number2
DOIs
StatePublished - Apr 1994
Externally publishedYes

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