Detection of Anisotropy in Cartilage Using 2H Double-Quantum-Filtered NMR-Spectroscopy

Yehuda Sharf, Uzi Eliav, Hadassah Shinar, Gil Navon

Research output: Contribution to journalArticlepeer-review


Double-quantum-filtered (DQF) NMR spectroscopy of I = 1 spin systems is a diagnostic tool for the detection of anisotropy in macroscopically disordered systems. For deuterium, this method reveals the presence of a residual quadrupolar interaction for D2O in bovine nasal cartilage. This tissue is not macroscopically ordered and the quadrupolar splitting is not resolved. Fitting the calculated spectral lineshapes to the experimental results was possible only when a distribution of the residual quadrupolar interaction, omega(q), was assumed. The series of DQF lineshapes obtained for different creation times in the DQF experiment could be fitted using a single set of three parameters: the average residual quadrupolar interaction ωq/2π = 110 Hz, its standard deviation Δωq/2π = 73 Hz, and the transverse relaxation rate of 63 s-1. Separate deuterium DQF measurements for the constituents of the cartilage, collagen, and chondroitin sulfate indicated that the DQF spectra of cartilage are the result of anisotropic motion of D2O due to binding to the fibrous collagen in the tissue.

Original languageEnglish
Pages (from-to)60-67
Number of pages8
JournalJournal of Magnetic Resonance - Series B
Issue number1
StatePublished - Apr 1995


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