Detection of amplified dna sequences in human tumor cell lines by fluorescence in situ hybridization

Irit Bar‐Am, Orna Mor, Yosef Shiloh, Lydia Avivi*, Herman Yeger

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

An unambiguous and rapid characterization of amplified DNA sequences in tumor cells is important for the understanding of neoplastic progression. This study was conducted to evaluate the potential of fluorescence in situ hybridization (FISH) to identify such amplified DNA sequences in human tumor cell lines. Applying this technique, we followed the metaphase location and interphase position of amplified DNA sequences corresponding to the SAMK, MYC, and MYCN genes in four cell lines derived from human tumors: two gastric carcinoma lines (KATO III and SNU‐16), a neuroblastoma (NUB‐7), and a neuroepithelioma (NUB‐20) line. In metaphase cells of KATO III, NUB‐7, and NUB‐20 lines, the amplified regions were clearly visible and easily identified at an intrachromosomal location: in KATO III and NUB‐7 at a terminal position and in NUB‐20 at an interstitial position. In SNU‐16, on the other hand, the amplified SAMK and MYC sequences were identified in extrachromosomal double minute chromosomes (DMs). In this line, the SAMK and MYC sequences were coamplified in the same cells and were colocated on the same DMs. FISH also allowed the identification of amplified DNA sequences in nondividing cells, enabling us to distinguish, at interphase, whether the amplification gave rise to intrachromosomal amplified regions (IARs) or to extrachromosomal DMs. The FISH technique also allowed us to determine at metaphase as well as at interphase the extent of amplification and the size of the IARs.

Original languageEnglish
Pages (from-to)314-320
Number of pages7
JournalGenes Chromosomes and Cancer
Volume4
Issue number4
DOIs
StatePublished - Jun 1992

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