Detection of α1 integrin in urine of patients with immunoglobulin a nephropathy

Jonathan Bank, Aharon Ben-David, Ram Doolman, Ben Ami Sela, Ilan Bank

Research output: Contribution to journalArticlepeer-review

Abstract

Background: The α1β1 integrin is a cell surface membrane heterodimer composed of noncovalently linked !1 and "1 polypeptides that is up-regulated on activated and proliferating mesangial cells.Methods: A double-sandwich enzyme-linked immunosorbent assay that detects !1 integrin in a specific and dosedependent manner at concentrations greater than 150 ng/mL was used to evaluate whether intact !1 polypeptides are secreted in the urine samples of 29 patients with various kidney diseases and in those of 5 healthy individuals.Results: α1 Integrin was detected in 8 of the 29 patients including 3 of 3 patients with biopsy-proven immunoglobulin A nephropathy and 3 of 3 clinically suspected but non-biopsy-proven immunoglobulin A nephropathy with evidence of active nephritis. No !1 integrins were found in samples of 5 healthy controls.Conclusions: !1 Integrin polypeptides can be detected in human urine, particularly in immunoglobulin A nephropathy.Further extensive studies are required to clarify the significance of secretion of !1 integrins in urine ofpatients with kidney disease. Copyright @ 2008 American Federation for Medical Research.

Original languageEnglish
Pages (from-to)581-586
Number of pages6
JournalJournal of Investigative Medicine
Volume56
Issue number3
DOIs
StatePublished - Mar 2008

Keywords

  • IgA nephropathy α1 integrin
  • Integrins
  • Urine
  • VLA-1

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