Detection and differentiation of bacteria by electrical bioimpedance spectroscopy

Rima Gnaim, Alexander Golberg, Julia Sheviryov, Boris Rubinsky, César A. González

Research output: Contribution to journalArticlepeer-review

Abstract

Detecting bacteria in samples and differentiating between Gram-negative and Gram-positive species is an important challenge, and the most common method, Gram staining, is very time consuming. The aim of this study was to evaluate the electrical bioimpedance spectroscopy (EBIS) technique as an inexpensive and practical tool for real-time detection of bacteria and differentiation between Gram-positive and Gram-negative species. The relevant sensitivity for differentiating between specieswas found in the magnitude and phase at frequencies of 158,489 and 5248 Hz, respectively, at a bacterial concentration of 1 μg/μl. Subsequently, the sensitivity was estimated as a function of bacterial concentration. Our results demonstrated that EBIS can potentially distinguish between presence and absence of bacteria as well as between different types of bacteria.

Original languageEnglish
Pages (from-to)27-36
Number of pages10
JournalBioTechniques
Volume69
Issue number1
DOIs
StatePublished - Apr 2020

Keywords

  • Bacteria
  • Electrical bioimpedance spectroscopy
  • Gram negative
  • Gram positive

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