Desorption of adherent bacteria from a solid hydrophobic surface by oil

Oils efficiently desorbed bacteria from a solid surface (polystyrene). Desorption was measured qualitatively by staining techniques, and quantitatively by following the outgrowth kinetics of cells remaining on the plastic surface following the washing procedure. Approximately 97% of Acinetobacter calcoaceticus RAG-1 cells adhering to a polystyrene cuvette were removed following two successive washings in the presence of olive oil.s Results suggest that oils may serve as potent, non-toxic desorbing agents to remove attached hydrophobic bacteria from surfaces.