TY - JOUR
T1 - Designing a QTL Mapping Study for Implementation in the Realized Collaborative Cross Genetic Reference Population
AU - Soller, Morris
AU - Abu-Toamih Atamni, Hanifa J.
AU - Binenbaum, Ilona
AU - Chatziioannou, Aristotelis
AU - Iraqi, Fuad A.
N1 - Publisher Copyright:
© 2019 John Wiley & Sons, Inc.
PY - 2019/12/1
Y1 - 2019/12/1
N2 - The Collaborative Cross (CC) mouse resource is a next-generation mouse genetic reference population (GRP) designed for high-resolution mapping of quantitative trait loci (QTL) of large effect affecting complex traits during health and disease. The CC resource consists of a set of 72 recombinant inbred lines (RILs) generated by reciprocal crossing of five classical and three wild-derived mouse founder strains. Complex traits are controlled by variations within multiple genes and environmental factors, and their mutual interactions. These traits are observed at multiple levels of the animals' systems, including metabolism, body weight, immune profile, and susceptibility or resistance to the development and progress of infectious or chronic diseases. Herein, we present general guidelines for design of QTL mapping experiments using the CC resource-along with full step-by-step protocols and methods that were implemented in our lab for the phenotypic and genotypic characterization of the different CC lines-for mapping the genes underlying host response to infectious and chronic diseases.
AB - The Collaborative Cross (CC) mouse resource is a next-generation mouse genetic reference population (GRP) designed for high-resolution mapping of quantitative trait loci (QTL) of large effect affecting complex traits during health and disease. The CC resource consists of a set of 72 recombinant inbred lines (RILs) generated by reciprocal crossing of five classical and three wild-derived mouse founder strains. Complex traits are controlled by variations within multiple genes and environmental factors, and their mutual interactions. These traits are observed at multiple levels of the animals' systems, including metabolism, body weight, immune profile, and susceptibility or resistance to the development and progress of infectious or chronic diseases. Herein, we present general guidelines for design of QTL mapping experiments using the CC resource-along with full step-by-step protocols and methods that were implemented in our lab for the phenotypic and genotypic characterization of the different CC lines-for mapping the genes underlying host response to infectious and chronic diseases.
KW - Collaborative Cross mouse model
KW - complex traits
KW - experimental design for QTL mapping
KW - genetic reference population
KW - quantitative trait loci
KW - statistical power
UR - http://www.scopus.com/inward/record.url?scp=85075461739&partnerID=8YFLogxK
U2 - 10.1002/cpmo.66
DO - 10.1002/cpmo.66
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AN - SCOPUS:85075461739
SN - 2161-2617
VL - 9
SP - e66
JO - Current protocols in mouse biology
JF - Current protocols in mouse biology
IS - 4
ER -