@article{7e1f45a74f9a4cc48c11263f302851a0,
title = "Design of SARS-CoV-2 hFc-Conjugated Receptor-Binding Domain mRNA Vaccine Delivered via Lipid Nanoparticles",
abstract = "Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been identified as the causal agent of COVID-19 and stands at the center of the current global human pandemic, with death toll exceeding one million. The urgent need for a vaccine has led to the development of various immunization approaches. mRNA vaccines represent a cell-free, simple, and rapid platform for immunization, and therefore have been employed in recent studies toward the development of a SARS-CoV-2 vaccine. Herein, we present the design of an mRNA vaccine, based on lipid nanoparticles (LNPs)-encapsulated SARS-CoV-2 human Fc-conjugated receptor-binding domain (RBD-hFc). Several ionizable lipids have been evaluated in vivo in a luciferase (luc) mRNA reporter assay, and two leading LNPs formulations have been chosen for the subsequent RBD-hFc mRNA vaccine strategy. Intramuscular administration of LNP RBD-hFc mRNA elicited robust humoral response, a high level of neutralizing antibodies and a Th1-biased cellular response in BALB/c mice. The data in the current study demonstrate the potential of these lipids as promising candidates for LNP-based mRNA vaccines in general and for a COVID19 vaccine in particular.",
keywords = "COVID-19, SARS-CoV-2, ionizable lipids, lipid nanoparticles, mRNA vaccine",
author = "Uri Elia and Srinivas Ramishetti and Ronit Rosenfeld and Niels Dammes and Erez Bar-Haim and Naidu, {Gonna Somu} and Efi Makdasi and Yfat Yahalom-Ronen and Hadas Tamir and Nir Paran and Ofer Cohen and Dan Peer",
note = "Publisher Copyright: {\textcopyright} ",
year = "2021",
month = jun,
day = "22",
doi = "10.1021/acsnano.0c10180",
language = "אנגלית",
volume = "15",
pages = "9627--9637",
journal = "ACS Nano",
issn = "1936-0851",
publisher = "American Chemical Society",
number = "6",
}