Design of a human synthetic combinatorial library of single-chain antibodies.

Limor Nahary; Itai Benhar

doi:10.1007/978-1-59745-554-1_3

Design of a human synthetic combinatorial library of single-chain antibodies.

Limor Nahary^*
, Itai Benhar

^*Corresponding author for this work

Biotechnology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Antibody libraries came into existence 15 years ago when the accumulating sequence data of immunoglobulin genes and the advent of the PCR technology made it possible to clone antibody gene repertoires. Phage display (most common) and additional display and screening technologies were applied to pan out desired binding specificities from antibody libraries. "Synthetic" or "semi-synthetic" libraries are from naïve, non-immunized source and considered to be a source for many different targets, including self-antigens. We describe here how to construct a large human synthetic single-chain Fv (scFv) antibody library displayed on phages, where in vivo-formed complementarity-determining regions (CDRs) are shuffled combinatorially onto germline-derived human variable-region frameworks.

Original language	English
Pages (from-to)	61-80, xiv
Journal	Methods in Molecular Biology
Volume	525
DOIs	https://doi.org/10.1007/978-1-59745-554-1_3
State	Published - 2009

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Design of a human synthetic combinatorial library of single-chain antibodies.

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