Design for a 10 keV multi-pass transmission electron microscope

Stewart A. Koppell*, Marian Mankos, Adam J. Bowman, Yonatan Israel, Thomas Juffmann, Brannon B. Klopfer, Mark A. Kasevich

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Multi-pass transmission electron microscopy (MPTEM) has been proposed as a way to reduce damage to radiation-sensitive materials. For the field of cryo-electron microscopy (cryo-EM), this would significantly reduce the number of projections needed to create a 3D model and would allow the imaging of lower-contrast, more heterogeneous samples. We have designed a 10 keV proof-of-concept MPTEM. The column features fast-switching gated electron mirrors which cause each electron to interrogate the sample multiple times. A linear approximation for the multi-pass contrast transfer function (CTF) is developed to explain how the resolution depends on the number of passes through the sample.

Original languageEnglish
Article number112834
JournalUltramicroscopy
Volume207
DOIs
StatePublished - Dec 2019
Externally publishedYes

Funding

FundersFunder number
Stanford Graduate Fellowship
National Science Foundation1656518
Gordon and Betty Moore Foundation

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