The interest in nylon and related polyamides as possible supports for the covalent attachment of enzymes stems from their high stability and relatively low cost. However, they suffer from a serious drawback in that only the terminal carboxyl and amino groups can be utilized for the covalent binding of proteins. Procedures by which the binding capacity of nylon could be increased by mild acid hydrolysis have been described recently by Hornby et al. This communication describes a method for introducing chemically reactive groups onto the nylon backbone, without causing a decrease in the mol. wt and thus impairing the mechanical properties of the final product. Details of the experimental study are presented in which various enzymes were mobilized on supports of various polyamides and their derivatives.
|Number of pages||8|
|State||Published - 1974|
|Event||Eng Found Conf on Enzyme Eng, 2nd, Proc - Henniker, NH, USA|
Duration: 5 Aug 1973 → 10 Aug 1973
|Conference||Eng Found Conf on Enzyme Eng, 2nd, Proc|
|City||Henniker, NH, USA|
|Period||5/08/73 → 10/08/73|