TY - JOUR
T1 - Deregulated expression of homeobox-containing genes, HOXB6, B8, C8, C9, and Cdx-1, in human colon cancer cell lines
AU - Vider, Ben Zion
AU - Zimber, Amazia
AU - Chastre, Eric
AU - Gespach, Christian
AU - Halperin, Marisa
AU - Mashiah, Pnina
AU - Yaniv, Abraham
AU - Gazit, Arnona
N1 - Funding Information:
This work was supported by grants from the Israel Science Foundation, founded by the Israel Academy of Science and Humanities, and from the United States-Israel Binational Science Foundation (BSF).
PY - 2000/6/7
Y1 - 2000/6/7
N2 - Previously we have demonstrated a reciprocal deregulation of various homeobox genes (HOXB6, B8, C8 and C9 vs Cdx-1) in human colorectal cancer (CRC). In the present study, using RT-PCR, we have investigated the expression pattern of these homeobox genes in various human colon cell lines, representing various stages of colon cancer progression and differentiation. Thus, we have tested polyposis coli Pc/AA adenoma cells, Caco-2, HT-29 and LS174T adenocarcinoma cell lines. All cell lines, except LS174T, demonstrated a pattern of deregulated homeobox gene expression which resembled that of CRC. In contrast, the pattern of expression of these genes in the highly oncogenic LS174T cells, as well as in Caco-2 cells transfected with activated Ha-ras or Polyoma middle T oncogene, resembled that of the normal mucosa. The reciprocal deregulation of HOX and Cdx-1 genes in CRC and in CRC-derived cell lines suggests a possible role in human CRC development. (C) 2000 Academic Press.
AB - Previously we have demonstrated a reciprocal deregulation of various homeobox genes (HOXB6, B8, C8 and C9 vs Cdx-1) in human colorectal cancer (CRC). In the present study, using RT-PCR, we have investigated the expression pattern of these homeobox genes in various human colon cell lines, representing various stages of colon cancer progression and differentiation. Thus, we have tested polyposis coli Pc/AA adenoma cells, Caco-2, HT-29 and LS174T adenocarcinoma cell lines. All cell lines, except LS174T, demonstrated a pattern of deregulated homeobox gene expression which resembled that of CRC. In contrast, the pattern of expression of these genes in the highly oncogenic LS174T cells, as well as in Caco-2 cells transfected with activated Ha-ras or Polyoma middle T oncogene, resembled that of the normal mucosa. The reciprocal deregulation of HOX and Cdx-1 genes in CRC and in CRC-derived cell lines suggests a possible role in human CRC development. (C) 2000 Academic Press.
UR - http://www.scopus.com/inward/record.url?scp=0034616647&partnerID=8YFLogxK
U2 - 10.1006/bbrc.2000.2804
DO - 10.1006/bbrc.2000.2804
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AN - SCOPUS:0034616647
SN - 0006-291X
VL - 272
SP - 513
EP - 518
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -
