Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4

Amnon Peled, Isabelle Petit, Orit Kollet, Michal Magid, Tanya Ponomaryov, Tamara Byk, Arnon Nagler, Herzl Ben-Hur, Ariel Many, Leonard Shultz, Ofer Lider, Ronen Alon, Dov Zipori, Tsvee Lapidot

Research output: Contribution to journalArticlepeer-review

Abstract

Stem cell homing and repopulation are not well understood. The chemokine stromal cell-derived factor-1 (SDF-1) and its receptor CXCR4 were found to be critical for murine bone marrow engraftment by human severe combined immunodeficient (SCID) repopulating stem cells. Treatment of human cells with antibodies to CXCR4 prevented engraftment. In vitro CXCR4-dependent migration to SDF-1 of CD34+CD38-(/low) cells correlated with in vivo engraftment and stem cell function. Stem cell factor and interleukin-6 induced CXCR4 expression on CD34+ cells, which potentiated migration to SDF-1 and engraftment in primary and secondary transplanted mice. Thus, up-regulation of CXCR4 expression may be useful for improving engraftment of repopulating stem cells in clinical transplantation.

Original languageEnglish
Pages (from-to)845-848
Number of pages4
JournalScience
Volume283
Issue number5403
DOIs
StatePublished - 5 Feb 1999

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