@article{a386236153db4688b25bd0b506aedcdd,
title = "Delta and kappa opioid receptors in eyestalk ganglia of a crustacean",
abstract = "Crustacean eyestalk ganglia are part of the protocerebrum and have been demonstrated to produce numerous neurohormones. 3H(2-D-Pen, 5-D-Pen)-enkephalin, 3H-(-)-ethylketocyclazocine and 3H(D-Ala2-NMePhe-Glyol5)-enkephalin were used as ligands for opioid receptors on neuronal membrane preparations of eyestalk ganglia under consideration of their stereospecific binding properties. In context with saturation binding isotherms, association and dissociation plots, we demonstrate here two opioid receptors: a delta-type receptor with high affinity (B(max) 68.5 fmol/mg protein, K(d) = 4.0 nM) and low affinity (B(max) 493 fmol/mg, K(d) = 83.6 nM) and a second receptor of kappa-type with B(max) of 3.1 pmol/mg protein and K(d) = 68.6 nM.",
keywords = "crustacea, enkephalin, eyestalk ganglion, opioid receptor subtype, radioligand binding",
author = "Joachim Hanke and Axel Willig and Uri Yinon and Jaros, {Peter P.}",
note = "Funding Information: This work was supported by grants from Lower Saxony to J.H. (Gradf{\"o}g 7/27/04), and to P.P.J. from the DFG (JA 397/2-2) and from the VW Stiftung (210.3-99-4/94). Dextrorphan was a generous gift of Hoffmann La Roche, Basel. We thank J. McReynolds and A. Sievers for critically reading the manuscript.",
year = "1997",
month = jan,
day = "9",
doi = "10.1016/S0006-8993(96)01114-6",
language = "אנגלית",
volume = "744",
pages = "279--284",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier B.V.",
number = "2",
}