Delayed activation of phospholipase D by gonadotropin-releasing hormone in a clonal pituitary gonadotrope cell line (αT3-1)

Einat Netiv; Mordechai Liscovitch; Zvi Naor

doi:10.1016/0014-5793(91)81396-P

Delayed activation of phospholipase D by gonadotropin-releasing hormone in a clonal pituitary gonadotrope cell line (αT3-1)

Einat Netiv, Mordechai Liscovitch, Zvi Naor^*

^*Corresponding author for this work

Biochemistry Molecular Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Stimulation of cultured pituitary cells from a gonadotrope lineage (αT3-1) by the gonadotropin-releasing hormone agonist analog [D-Trp⁶]GnRH (GnRH-A) resulted in a manifold increase in accumulation of phosphatidylethanol, a specific product of phospholipase D phosphatidyl transferase activity when ethanol is the phosphatidyl group acceptor. Levels of the natural lipid product of phospholipase D, phosphatidic acid, were increased 2-3-fold. Activation of phospholipase D by GnRH-A was dose- and time-dependent and was blocked by a GnRH receptor antagonist [D-pClPhe²,D-Trp^3.6]GnRH. GnRH-A stimulated phospholipase D activity after a lag of 1-2 min. We conclude that in αT3-1 gonadotropes GnRH receptor occupancy results in delayed activation of phospholipase D which could participate in late phases of gonadotrope regulation by the neurohormone.

Original language	English
Pages (from-to)	107-109
Number of pages	3
Journal	FEBS Letters
Volume	295
Issue number	1-3
DOIs	https://doi.org/10.1016/0014-5793(91)81396-P
State	Published - 16 Dec 1991

Keywords

Diacylglycerol
GnRH
Phosphatidic acid
Phosphatidylcholine
Protein kinase C
Signal transduction

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10.1016/0014-5793(91)81396-P

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title = "Delayed activation of phospholipase D by gonadotropin-releasing hormone in a clonal pituitary gonadotrope cell line (αT3-1)",

abstract = "Stimulation of cultured pituitary cells from a gonadotrope lineage (αT3-1) by the gonadotropin-releasing hormone agonist analog [D-Trp6]GnRH (GnRH-A) resulted in a manifold increase in accumulation of phosphatidylethanol, a specific product of phospholipase D phosphatidyl transferase activity when ethanol is the phosphatidyl group acceptor. Levels of the natural lipid product of phospholipase D, phosphatidic acid, were increased 2-3-fold. Activation of phospholipase D by GnRH-A was dose- and time-dependent and was blocked by a GnRH receptor antagonist [D-pClPhe2,D-Trp3.6]GnRH. GnRH-A stimulated phospholipase D activity after a lag of 1-2 min. We conclude that in αT3-1 gonadotropes GnRH receptor occupancy results in delayed activation of phospholipase D which could participate in late phases of gonadotrope regulation by the neurohormone.",

keywords = "Diacylglycerol, GnRH, Phosphatidic acid, Phosphatidylcholine, Protein kinase C, Signal transduction",

author = "Einat Netiv and Mordechai Liscovitch and Zvi Naor",

note = "Funding Information: ,l~~rro~rl~rl~~t,rr,WlrsC: thank Dr. kilnda L. Mellon for kindly providing the (zT3-I cell lint. This work was supported by the Gcrman-lsracli Foundation for Rcscarch and Dcsclopmcnc (Z.N.). the Fund for Basic Rcscarch adminiswrcd by the isracl Academy of Scicnccs, Ihc Irwin Green Rcscarch Fund in lhc Ncuroscicnccs and Ihc Chief Scicnlisl{\textquoteright}s Office. Minislry of Health. krusdh (M.L.). M.L. is a rccipisnl of an Yigal Allon Fellowship and the incumbcni of the Shloimo and Michla Tomarin Career Dcvclopmcnl Chair in Mcm-branc Physiology.",

year = "1991",

month = dec,

day = "16",

doi = "10.1016/0014-5793(91)81396-P",

language = "אנגלית",

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pages = "107--109",

journal = "FEBS Letters",

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T1 - Delayed activation of phospholipase D by gonadotropin-releasing hormone in a clonal pituitary gonadotrope cell line (αT3-1)

AU - Netiv, Einat

AU - Liscovitch, Mordechai

AU - Naor, Zvi

N1 - Funding Information: ,l~~rro~rl~rl~~t,rr,WlrsC: thank Dr. kilnda L. Mellon for kindly providing the (zT3-I cell lint. This work was supported by the Gcrman-lsracli Foundation for Rcscarch and Dcsclopmcnc (Z.N.). the Fund for Basic Rcscarch adminiswrcd by the isracl Academy of Scicnccs, Ihc Irwin Green Rcscarch Fund in lhc Ncuroscicnccs and Ihc Chief Scicnlisl’s Office. Minislry of Health. krusdh (M.L.). M.L. is a rccipisnl of an Yigal Allon Fellowship and the incumbcni of the Shloimo and Michla Tomarin Career Dcvclopmcnl Chair in Mcm-branc Physiology.

PY - 1991/12/16

Y1 - 1991/12/16

N2 - Stimulation of cultured pituitary cells from a gonadotrope lineage (αT3-1) by the gonadotropin-releasing hormone agonist analog [D-Trp6]GnRH (GnRH-A) resulted in a manifold increase in accumulation of phosphatidylethanol, a specific product of phospholipase D phosphatidyl transferase activity when ethanol is the phosphatidyl group acceptor. Levels of the natural lipid product of phospholipase D, phosphatidic acid, were increased 2-3-fold. Activation of phospholipase D by GnRH-A was dose- and time-dependent and was blocked by a GnRH receptor antagonist [D-pClPhe2,D-Trp3.6]GnRH. GnRH-A stimulated phospholipase D activity after a lag of 1-2 min. We conclude that in αT3-1 gonadotropes GnRH receptor occupancy results in delayed activation of phospholipase D which could participate in late phases of gonadotrope regulation by the neurohormone.

AB - Stimulation of cultured pituitary cells from a gonadotrope lineage (αT3-1) by the gonadotropin-releasing hormone agonist analog [D-Trp6]GnRH (GnRH-A) resulted in a manifold increase in accumulation of phosphatidylethanol, a specific product of phospholipase D phosphatidyl transferase activity when ethanol is the phosphatidyl group acceptor. Levels of the natural lipid product of phospholipase D, phosphatidic acid, were increased 2-3-fold. Activation of phospholipase D by GnRH-A was dose- and time-dependent and was blocked by a GnRH receptor antagonist [D-pClPhe2,D-Trp3.6]GnRH. GnRH-A stimulated phospholipase D activity after a lag of 1-2 min. We conclude that in αT3-1 gonadotropes GnRH receptor occupancy results in delayed activation of phospholipase D which could participate in late phases of gonadotrope regulation by the neurohormone.

KW - Diacylglycerol

KW - GnRH

KW - Phosphatidic acid

KW - Phosphatidylcholine

KW - Protein kinase C

KW - Signal transduction

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JO - FEBS Letters

JF - FEBS Letters

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ER -

Delayed activation of phospholipase D by gonadotropin-releasing hormone in a clonal pituitary gonadotrope cell line (αT3-1)

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Keywords

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