TY - JOUR
T1 - Defensin interferes with the activation of neutrophil NADPH oxidase a cell-free system
AU - Tal, Tal
AU - Aviram, Irit
PY - 1993/10/29
Y1 - 1993/10/29
N2 - NHP-1, a member of a family of microbicidal cationic, cystine-rich polypeptides abundant in the azurophilic granules of polymorphonuclear leukocytes (PMN) interfered with detergent-induced cell-free activation of superoxide-producing NADPH oxidase. IC50 of 3 μM has been evaluated for this dose dependent effect of defensin. Preactivated oxidase was not affected by HNP-1. The inhibitory effect could be relieved by raising the concentration of the cytosol or by the addition of a partially purified p47-phox. NADPH oxidase activated in the presence of HNP-1 exhibited an increased affinity for NADPH.
AB - NHP-1, a member of a family of microbicidal cationic, cystine-rich polypeptides abundant in the azurophilic granules of polymorphonuclear leukocytes (PMN) interfered with detergent-induced cell-free activation of superoxide-producing NADPH oxidase. IC50 of 3 μM has been evaluated for this dose dependent effect of defensin. Preactivated oxidase was not affected by HNP-1. The inhibitory effect could be relieved by raising the concentration of the cytosol or by the addition of a partially purified p47-phox. NADPH oxidase activated in the presence of HNP-1 exhibited an increased affinity for NADPH.
UR - http://www.scopus.com/inward/record.url?scp=0027378196&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1993.2297
DO - 10.1006/bbrc.1993.2297
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AN - SCOPUS:0027378196
SN - 0006-291X
VL - 196
SP - 636
EP - 641
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -