Decreased expression of the p21ras stimulatory factor hSOS in PBMC from inactive SLE patients

M. J. Rapoport*, A. Mor, M. Amit, R. Rosenberg, Y. Ramot, A. Mizrachi, A. J. Wysenbeek

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Expression of the p21ras protooncogene is reported to be increased in animal models and in patients with SLE. However, the expression of p21ras regulatory elements has not been determined. We determined the expression of p21ras, and its regulatory elements p120-ras-GAP and hSOS, in PBMC of 10 patients with inactive SLE (mean SLEDAI score 1.8 ± 0.53) and 10 age- and sex-matched healthy controls. No difference was found between the two groups in the levels of p21ras (3760 ± 513 and 3367 ± 335, P = 0.25) and ras-GAP (1048 ± 261 and 1534 ± 247, P = 0.11) in patients and controls, respectively. In contrast, levels of hSOS were significantly decreased in patients as compared to controls: 955 ± 218 and 2306 ± 327, P = 0.002, respectively. The mitogen induced proliferative response was comparable in the two groups: SI 20.8 ± 4.2 and 15.03 ± 4.91, P = 0.135, in patients and controls, respectively. Taken together, our data demonstrate that nonactive SLE patients are characterized by reduced hSOS expression and underscore the need for a comprehensive evaluation of p21ras pathway in these patients.

Original languageEnglish
Pages (from-to)24-28
Number of pages5
JournalLupus
Volume8
Issue number1
DOIs
StatePublished - 1999

Keywords

  • Human
  • PBMC
  • Ras-GAP
  • SLE
  • hSOS
  • p21ras

Fingerprint

Dive into the research topics of 'Decreased expression of the p21ras stimulatory factor hSOS in PBMC from inactive SLE patients'. Together they form a unique fingerprint.

Cite this