Decrease of intracellular fluorescein fluorescence polarization (IFFP) in human peripheral blood lymphocytes undergoing stimulation with phytohaemagglutinin (PHA), concanavalin A (ConA), pokeweed mitogen (PWM) and anti-CD3 antibody

Avi Eisenthal, Oleg Marder, Dalia Dotan, Shoshana Baron, Beatriz Lifschitz-Mercer, Samario Chaitchik, Reuven Tirosh, Arye Weinreb, Motti Deutsch

Research output: Contribution to journalArticlepeer-review

Abstract

In the present study we describe the induction of changes in intracellular fluorescein fluorescence polarization (IFFP) in lymphocytes undergoing activation with a variety of stimulants. These stimulants included the lectins phytohaemagglutinin (PHA), concanavalin (ConA), pokeweed mitogen (PWM) and anti-CD3 antibody. Changes in IFFP were detected in individual cells using the Cellscan apparatus. Our results show that by employing mitogenic concentrations of PHA, as revealed in a [3H]-thymidine incorporation assay, a decrease in the IFFP in human peripheral blood lymphocytes (PBL) occurred within 40 min. ConA and anti-CD3 affected similarly IFFP, whereas PWM, a B lymphocyte lectin, had no effect on IFFP at the concentrations employed. Kinetic analysis revealed that changes in IFFP occurred within 20-40 min after exposure to the stimulants and lasted for 24 h. Our results show that stimulants which activate CD3+ lymphocytes caused immediate changes in IFFP, in an enriched population of human PBL. The possible mechanisms involved in IFFP modulation following exposure to selected stimulants are discussed.

Original languageEnglish
Pages (from-to)145-150
Number of pages6
JournalBiology of the Cell
Volume86
Issue number2-3
DOIs
StatePublished - 1996

Keywords

  • Activation
  • Anti-CD3
  • Cytoplasma
  • Lymphocytes
  • Mitogen

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