TY - JOUR
T1 - Decelerated rate of dendrite outgrowth from dopaminergic neurons in primary cultures from brains of hypoxanthine phosphoribosyltransferase-deficient knockout mice
AU - Boer, Pnina
AU - Brosh, Sara
AU - Wasserman, Lina
AU - Hammel, Ilan
AU - Zoref-Shani, Esther
AU - Sperling, Oded
N1 - Funding Information:
The authors gratefully acknowledge the help of Dr N. Kariv, of the Animal Facility of the Sackler Faculty, in breeding the mice. The research was partly supported by a grant (3392) from the Chief Scientist of the Ministry of Health of Israel.
PY - 2001/4/27
Y1 - 2001/4/27
N2 - Lesch-Nyhan syndrome (LNS), caused by the complete deficiency of hypoxanthine phosphoribosyltransferase (HPRT), is characterized by a neurological deficit, the etiology of which is still unclear. Evidence has accumulated indicating that it reflects dopamine deficiency associated with defective arborization of dopaminergic dendrites. We monitored the differentiation in vitro of dopaminergic neurons, cultured from HPRT-deficient knockout mice. The HPRT-deficient dopaminergic neurons exhibited a decelerated rate of outgrowth of dendrites in comparison to that of control neurons resulting, after 8 days in culture, in 32% smaller average total length of dendrites per neuron (P < 0.025). The results suggest that the abnormal dendrite outgrowth in LNS reflects a defective developmental process.
AB - Lesch-Nyhan syndrome (LNS), caused by the complete deficiency of hypoxanthine phosphoribosyltransferase (HPRT), is characterized by a neurological deficit, the etiology of which is still unclear. Evidence has accumulated indicating that it reflects dopamine deficiency associated with defective arborization of dopaminergic dendrites. We monitored the differentiation in vitro of dopaminergic neurons, cultured from HPRT-deficient knockout mice. The HPRT-deficient dopaminergic neurons exhibited a decelerated rate of outgrowth of dendrites in comparison to that of control neurons resulting, after 8 days in culture, in 32% smaller average total length of dendrites per neuron (P < 0.025). The results suggest that the abnormal dendrite outgrowth in LNS reflects a defective developmental process.
KW - Dendrite outgrowth
KW - Dopaminergic neurons
KW - HPRT-deficient mice
KW - Hypoxanthine phosphoribosyltransferase (HPRT)
KW - Lesch-Nyhan syndrome
KW - Tyrosine hydroxylase positive neurons
UR - http://www.scopus.com/inward/record.url?scp=0035957854&partnerID=8YFLogxK
U2 - 10.1016/S0304-3940(01)01716-5
DO - 10.1016/S0304-3940(01)01716-5
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AN - SCOPUS:0035957854
VL - 303
SP - 45
EP - 48
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -