Cytotoxicity of B72.3XOKT3 bispecific antibody recognizing human colon cancer

Baruch Shpitz; Hartley Stern; Jas S. Sandhu; Raymond M. Reilley; Michael B. Tropak; Gwen Jansz; Jennis Xu; Steven Gallinger

doi:10.1006/jsre.1996.0093

Cytotoxicity of B72.3XOKT3 bispecific antibody recognizing human colon cancer

Baruch Shpitz^*
, Hartley Stern
, Jas S. Sandhu
, Raymond M. Reilley
, Michael B. Tropak
, Gwen Jansz
, Jennis Xu
, Steven Gallinger

^*Corresponding author for this work

University of Toronto
S. Lunenfield Research Institute

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Bispecific monoclonal antibodies can be used to redirect peripheral blood lymphocytes against tumor cells. In the present study, a murine bispecific monoclonal antibody was developed using somatic hybridization. The antibody has two different binding arms: one arm directed against human CD3 receptor expressed on T-lymphocytes and the other against tumor associated glycoprotein TAG-72, expressed on human carcinomas, such as colon, breast, and pancreas. Partially purified antibody was capable of inducing human T- cell proliferation and preventing growth of colon cancer cell line in nu/nu mice in a tumor neutralization assay.

Original language	English
Pages (from-to)	134-138
Number of pages	5
Journal	Journal of Surgical Research
Volume	61
Issue number	1
DOIs	https://doi.org/10.1006/jsre.1996.0093
State	Published - 15 Feb 1996
Externally published	Yes

Funding

Funders
Lederman Cancer Research Fund

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1006/jsre.1996.0093

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title = "Cytotoxicity of B72.3XOKT3 bispecific antibody recognizing human colon cancer",

abstract = "Bispecific monoclonal antibodies can be used to redirect peripheral blood lymphocytes against tumor cells. In the present study, a murine bispecific monoclonal antibody was developed using somatic hybridization. The antibody has two different binding arms: one arm directed against human CD3 receptor expressed on T-lymphocytes and the other against tumor associated glycoprotein TAG-72, expressed on human carcinomas, such as colon, breast, and pancreas. Partially purified antibody was capable of inducing human T- cell proliferation and preventing growth of colon cancer cell line in nu/nu mice in a tumor neutralization assay.",

author = "Baruch Shpitz and Hartley Stern and Sandhu, \{Jas S.\} and Reilley, \{Raymond M.\} and Tropak, \{Michael B.\} and Gwen Jansz and Jennis Xu and Steven Gallinger",

note = "Funding Information: 1Supported by Lederman Cancer Research Fund and American Physician Fellowship.",

year = "1996",

month = feb,

day = "15",

doi = "10.1006/jsre.1996.0093",

language = "אנגלית",

volume = "61",

pages = "134--138",

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T1 - Cytotoxicity of B72.3XOKT3 bispecific antibody recognizing human colon cancer

AU - Shpitz, Baruch

AU - Stern, Hartley

AU - Sandhu, Jas S.

AU - Reilley, Raymond M.

AU - Tropak, Michael B.

AU - Jansz, Gwen

AU - Xu, Jennis

AU - Gallinger, Steven

N1 - Funding Information: 1Supported by Lederman Cancer Research Fund and American Physician Fellowship.

PY - 1996/2/15

Y1 - 1996/2/15

N2 - Bispecific monoclonal antibodies can be used to redirect peripheral blood lymphocytes against tumor cells. In the present study, a murine bispecific monoclonal antibody was developed using somatic hybridization. The antibody has two different binding arms: one arm directed against human CD3 receptor expressed on T-lymphocytes and the other against tumor associated glycoprotein TAG-72, expressed on human carcinomas, such as colon, breast, and pancreas. Partially purified antibody was capable of inducing human T- cell proliferation and preventing growth of colon cancer cell line in nu/nu mice in a tumor neutralization assay.

AB - Bispecific monoclonal antibodies can be used to redirect peripheral blood lymphocytes against tumor cells. In the present study, a murine bispecific monoclonal antibody was developed using somatic hybridization. The antibody has two different binding arms: one arm directed against human CD3 receptor expressed on T-lymphocytes and the other against tumor associated glycoprotein TAG-72, expressed on human carcinomas, such as colon, breast, and pancreas. Partially purified antibody was capable of inducing human T- cell proliferation and preventing growth of colon cancer cell line in nu/nu mice in a tumor neutralization assay.

UR - https://www.scopus.com/pages/publications/0030040898

U2 - 10.1006/jsre.1996.0093

DO - 10.1006/jsre.1996.0093

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AN - SCOPUS:0030040898

SN - 0022-4804

VL - 61

SP - 134

EP - 138

JO - Journal of Surgical Research

JF - Journal of Surgical Research

IS - 1

ER -

Cytotoxicity of B72.3XOKT3 bispecific antibody recognizing human colon cancer

Abstract

Funding

UN SDGs

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