Cytokines in mice treated with amphotericin B-intralipid

Y. Shadkchan, Y. Keisari, Esther Segal*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Amphotericin B (AMB) intralipid (IL) admixtures (AMB-IL) are composed of components approved for clinical use and are commercially available at low cost. They are stable and exhibit in-vitro and in-vivo efficacy against Candida infections, as well as resulting in significantly reduced toxicity in comparison with that of conventionally administered amphotericin B. We examined the production of cytokines in uninfected mice treated with AMB or AMB-IL, as evaluated by expression of mRNA corresponding to the cytokines. Expression was measured by intensity of bands in comparison to the intensity of β-actin control bands, with the latter assigned an arbitrary standard value of 100% and other bands measured in relative percentages. We found that both in naïve and compromised mice, AMB treatment caused significantly greater production of the pro-inflammatory cytokines tumour necrosis factor alpha (TNF-γ) and interleukin 1 beta (IL-1β) than was seen in animals treated with AMB-IL or with another lipid AMB formulation, AmBisome. We hypothesize that the superior tolerance for the AMB-IL admixtures, as compared with conventional AMB, might derive from the reduced expression of the pro-inflammatory cytokines. TNF-α and IL-1β, which mediate many potentially adverse pathophysiological events similar to those seen as side-effects of AMB usage.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalMedical Mycology
Volume42
Issue number2
DOIs
StatePublished - Apr 2004

Keywords

  • Amphotericin B
  • Cytokines
  • Intralipid
  • Mice

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