TY - JOUR
T1 - Cytokines in experimental autoimmune vasculitis
T2 - Evidence for a Th2 type response
AU - Tomer, Y.
AU - Barak, V.
AU - Gilburd, B.
AU - Shoenfeld, Y.
PY - 1999
Y1 - 1999
N2 - Objective: To investigate the pathogenic role of cytokines in the development of experimental autoimmune vasculitis. Methods: BALB/c mice were immunized with human IgG-ANCA from a patient with WG. Control mice were immunized with normal human IgG. Levels of mouse IgG-ANCA and other autoantibodies were determined. The mice lungs and kidneys were examined for the development of vasculitis. Levels of interleukin-1β (IL-1β), IL-2, IL- 4, IL-6, interferon γ (IFNγ) and TNFα were determined by ELISA two weeks after immunization of the mice. Results: Mice immunized with human IgG-ANCA developed anti-human IgG-ANCA (= Ab2) and anti-anti-human IgG-ANCA (mouse IgG-ANCA = Ab3), while the controls did not develop these antibodies. The mice that were immunized with human IgG-ANCA developed perivascular mononuclear cell infiltrates in the lungs, suggesting vasculitis. Levels of IL-4, IL-6 and TNFα but not IL-1β, IL-2 and IFNγ were significantly elevated in the mice 2 weeks after immunization with IgG-ANCA. Conclusion: Our results suggest a pathogenic role for IL-4, IL-6 and TNFα in the initiation phase of autoimmune vasculitis. This suggests that a Th2 type immune response is responsible for the initiation of experimental autoimmune lung vasculitis, similar to Wegener's granulomatosis in humans.
AB - Objective: To investigate the pathogenic role of cytokines in the development of experimental autoimmune vasculitis. Methods: BALB/c mice were immunized with human IgG-ANCA from a patient with WG. Control mice were immunized with normal human IgG. Levels of mouse IgG-ANCA and other autoantibodies were determined. The mice lungs and kidneys were examined for the development of vasculitis. Levels of interleukin-1β (IL-1β), IL-2, IL- 4, IL-6, interferon γ (IFNγ) and TNFα were determined by ELISA two weeks after immunization of the mice. Results: Mice immunized with human IgG-ANCA developed anti-human IgG-ANCA (= Ab2) and anti-anti-human IgG-ANCA (mouse IgG-ANCA = Ab3), while the controls did not develop these antibodies. The mice that were immunized with human IgG-ANCA developed perivascular mononuclear cell infiltrates in the lungs, suggesting vasculitis. Levels of IL-4, IL-6 and TNFα but not IL-1β, IL-2 and IFNγ were significantly elevated in the mice 2 weeks after immunization with IgG-ANCA. Conclusion: Our results suggest a pathogenic role for IL-4, IL-6 and TNFα in the initiation phase of autoimmune vasculitis. This suggests that a Th2 type immune response is responsible for the initiation of experimental autoimmune lung vasculitis, similar to Wegener's granulomatosis in humans.
KW - Autoimmunity
KW - Cytokines
KW - Interleukin
KW - Vasculitis
UR - http://www.scopus.com/inward/record.url?scp=0032857042&partnerID=8YFLogxK
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AN - SCOPUS:0032857042
SN - 0392-856X
VL - 17
SP - 521
EP - 526
JO - Clinical and Experimental Rheumatology
JF - Clinical and Experimental Rheumatology
IS - 5
ER -