Cytokine network in nonresponding chronic hepatitis C patients with genotype 1: Role of triple therapy with interferon alpha, ribavirin, and ursodeoxycholate

Manuela G. Neuman*, Laurence M. Blendis, Neil H. Shear, Izabella M. Malkiewicz, Asma Ibrahim, Gady G. Katz, Danny Sapir, Zamir Halpern, Shlomo Brill, Hava Peretz, Sophie Magazinik, Fred M. Konikoff

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: (i) to characterize the profile of tumor necrosis factor alpha (TNF α), interleukin-6 (IL-6), IL 10, Fas-ligand and transforming growth factor beta (TGF β), chronic hepatitis C (HCV) patients with genotype 1; (ii) to determine the influence of triple therapy (TT) with interferon α (IFN α) + ribavirin + ursodeoxycholic acid on these cytokines and (iii) to establish the relationship between the pro-inflammatory cytokines and the outcome of treatment. Design and Methods: 22 patients infected with HCV - genotype 1 a/b and non responsive to IFN-α monotherapy were enrolled in the TT. The controls were 49 HCV naïve patients with genotype 1 a/b. Cytokine levels were measured using enzyme-linked immunosorbent assay (ELISA). Results: The baseline TNF α values (pg/mL) in the sustained responders (SRs) (63±3) were significantly lower than non-responders (NRs) (140±16) (p < 0.001). Baseline Fas (ng/mL) levels were also lower in SRs (4.3±0.2) than NRs (5.4±0.4) (p < 0.05). Conclusions: Fas and TNF α may be used as serological markers of inflammation and effectiveness of therapy.

Original languageEnglish
Pages (from-to)183-188
Number of pages6
JournalClinical Biochemistry
Volume34
Issue number3
DOIs
StatePublished - 2001

Keywords

  • Chronic hepatitis C
  • Fas
  • Genotype 1
  • Interferon alpha
  • Non-responders
  • Ribavirin
  • Tumor necrosis factor alpha
  • Ursodeoxycholic acid

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