Abstract
The specific binding of anions to oxidized horse‐heart cytochrome c has been studied by two methods: measuring the effect of ion concentration on the redox potential, and directly by gel nitration. The anions investigated were: chloride, phosphate, ADP and sulfanilate. The binding constants estimated are in the range of 1–10 mM−1, and the number of sites per protein molecule are 1 to 2. The specificity of anion binding to the oxidized protein is demonstrated.
Original language | English |
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Pages (from-to) | 500-505 |
Number of pages | 6 |
Journal | European Journal of Biochemistry |
Volume | 32 |
Issue number | 3 |
DOIs | |
State | Published - Feb 1973 |