The specific binding of anions to oxidized horse‐heart cytochrome c has been studied by two methods: measuring the effect of ion concentration on the redox potential, and directly by gel nitration. The anions investigated were: chloride, phosphate, ADP and sulfanilate. The binding constants estimated are in the range of 1–10 mM−1, and the number of sites per protein molecule are 1 to 2. The specificity of anion binding to the oxidized protein is demonstrated.
|Number of pages||6|
|Journal||European Journal of Biochemistry|
|State||Published - Feb 1973|