TY - JOUR
T1 - Cutting edge
T2 - Distinct B cell repertoires characterize patients with mild and severe COVID-19
AU - Hoehn, Kenneth B.
AU - Ramanathan, Palaniappan
AU - Unterman, Avraham
AU - Sumida, Tomokazu S.
AU - Asashima, Hiromitsu
AU - Hafler, David A.
AU - Kaminski, Naftali
AU - Dela Cruz, Charles S.
AU - Sealfon, Stuart C.
AU - Bukreyev, Alexander
AU - Kleinstein, Steven H.
N1 - Publisher Copyright:
Copyright © 2021 by The American Association of Immunologists, Inc. 0022-1767/21/$37.50
PY - 2021/6/15
Y1 - 2021/6/15
N2 - Protective immunity against COVID-19 likely depends on the production of SARS-CoV-2-specific plasma cells and memory B cells postinfection or postvaccination. Previous work has found that germinal center reactions are disrupted in severe COVID-19. This may adversely affect long-term immunity against reinfection. Consistent with an extrafollicular B cell response, patients with severe COVID-19 have elevated frequencies of clonally expanded, class-switched, unmutated plasmablasts. However, it is unclear whether B cell populations in individuals with mild COVID-19 are similarly skewed. In this study, we use single-cell RNA sequencing of B cells to show that in contrast to patients with severe COVID-19, subjects with mildly symptomatic COVID-19 have B cell repertoires enriched for clonally diverse, somatically hypermutated memory B cells ~30 d after the onset of symptoms. This provides evidence that B cell responses are less disrupted in mild COVID-19 and result in the production of memory B cells.
AB - Protective immunity against COVID-19 likely depends on the production of SARS-CoV-2-specific plasma cells and memory B cells postinfection or postvaccination. Previous work has found that germinal center reactions are disrupted in severe COVID-19. This may adversely affect long-term immunity against reinfection. Consistent with an extrafollicular B cell response, patients with severe COVID-19 have elevated frequencies of clonally expanded, class-switched, unmutated plasmablasts. However, it is unclear whether B cell populations in individuals with mild COVID-19 are similarly skewed. In this study, we use single-cell RNA sequencing of B cells to show that in contrast to patients with severe COVID-19, subjects with mildly symptomatic COVID-19 have B cell repertoires enriched for clonally diverse, somatically hypermutated memory B cells ~30 d after the onset of symptoms. This provides evidence that B cell responses are less disrupted in mild COVID-19 and result in the production of memory B cells.
UR - http://www.scopus.com/inward/record.url?scp=85108659181&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.2100135
DO - 10.4049/jimmunol.2100135
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C2 - 34049971
AN - SCOPUS:85108659181
SN - 0022-1767
VL - 206
SP - 2785
EP - 2790
JO - Journal of Immunology
JF - Journal of Immunology
IS - 12
ER -
