TY - JOUR
T1 - Curcumin
T2 - A potential radio-enhancer in head and neck cancer
AU - Khafif, Avi
AU - Lev-Ari, Shahar
AU - Vexler, Akiva
AU - Barnea, Itai
AU - Starr, Alex
AU - Karaush, Vicki
AU - Haif, Sasha
AU - Ben-Yosef, Rami
PY - 2009/10
Y1 - 2009/10
N2 - Objectives/Hypothesis: To investigate whether curcumin enhances the cytotoxic effect of radiotherapy in head and neck squamous cell carcinoma (HNSCC). Methods: HNSCC cell lines SCC-1, SCC-9, KB, as well as A431 cell line were treated with curcumin, irradiation, or their combination. Cell viability was evaluated by XTT assay. Cyclooxygenase-2 (COX-2), epithelial growth factor receptor (EGFR), and p-Erk1/2 were measured by Western blot analysis. CD-1 athymic nude mice with orthotopic implanted SCC-1 cells, were treated with control diet, curcumin containing diet, local single-dose radiation, or combination. Results: Curcumin (IC50 range, 15-22 lM) and radiation inhibited cell viability in all cell lines were tested. The combination of curcumin and radiation resulted in additive effect. Curcumin decreased COX-2 expression and inhibited phosphorylation of EGFR in SCC-1 cells. In tumor-bearing mice the combination regimen showed a decrease in both tumor weight (25%, P = .09) and tumor size (15%, P = .23) compared to the nontreated mice. Conclusions: Curcumin inhibited HNSCC cell growth and augmented the effect of radiation in vitro and in vivo. A possible mechanism is inhibition of COX-2 expression and EGFR phosphorylation. Head and neck squamous cell carcinoma, curcumin, radiation, cyclooxygenase-2, epithelial growth factor receptor.
AB - Objectives/Hypothesis: To investigate whether curcumin enhances the cytotoxic effect of radiotherapy in head and neck squamous cell carcinoma (HNSCC). Methods: HNSCC cell lines SCC-1, SCC-9, KB, as well as A431 cell line were treated with curcumin, irradiation, or their combination. Cell viability was evaluated by XTT assay. Cyclooxygenase-2 (COX-2), epithelial growth factor receptor (EGFR), and p-Erk1/2 were measured by Western blot analysis. CD-1 athymic nude mice with orthotopic implanted SCC-1 cells, were treated with control diet, curcumin containing diet, local single-dose radiation, or combination. Results: Curcumin (IC50 range, 15-22 lM) and radiation inhibited cell viability in all cell lines were tested. The combination of curcumin and radiation resulted in additive effect. Curcumin decreased COX-2 expression and inhibited phosphorylation of EGFR in SCC-1 cells. In tumor-bearing mice the combination regimen showed a decrease in both tumor weight (25%, P = .09) and tumor size (15%, P = .23) compared to the nontreated mice. Conclusions: Curcumin inhibited HNSCC cell growth and augmented the effect of radiation in vitro and in vivo. A possible mechanism is inhibition of COX-2 expression and EGFR phosphorylation. Head and neck squamous cell carcinoma, curcumin, radiation, cyclooxygenase-2, epithelial growth factor receptor.
KW - Curcumin
KW - Cyclooxygenase-2
KW - Epithelial growth factor receptor
KW - Head and neck squamous cell carcinoma
KW - Radiation
UR - http://www.scopus.com/inward/record.url?scp=70349939454&partnerID=8YFLogxK
U2 - 10.1002/lary.20582
DO - 10.1002/lary.20582
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C2 - 19655336
AN - SCOPUS:70349939454
SN - 0023-852X
VL - 119
SP - 2019
EP - 2026
JO - Laryngoscope
JF - Laryngoscope
IS - 10
ER -