TY - JOUR
T1 - Culture of human granulosa cells from an in vitro fertilization program
T2 - Effects of extracellular matrix on morphology and cyclic adenosine 3',5' monophosphate production
AU - Furman, A.
AU - Rotmensch, S.
AU - Dor, J.
AU - Venter, A.
AU - Mashiach, S.
AU - Vlodavsky, I.
AU - Amsterdam, A.
N1 - Funding Information:
Acknowledgments. We thank Dr. Edwina Rudak for providing us with the freshly harvested GCs. The skillful secretarial assistance of Mrs. MaIka Kopelowitz is gratefully acknowledged. Some of the ECM-coated dishes were kindly provided by IBT, International Biotechnologies, Kiryat Hadassah, Jerusalem, Israel. A. Amsterdam is the incumbent of the Joyce and Ben B. Eisenberg Professorial Chair in Molecular Endocrinology and Cancer Research. This work was supported by grants from the Rockefeller Foundation (to the Department of Hormone Research, Weizmann Institute of Science) and from the NIH (to 1. Vlodavsky).
Funding Information:
Received July 9, 1985; revised and accepted May 22, 1986. *Supported by a grant from the Rockefeller Foundation and by grant CA 30289 from the National Institutes of Health. tDepartment of Hormone Research, The Weizmann Institute of Science. :j:Department of Obstetrics and Gynecology, Chaim Sheba Medical Center. §Department of Radiation and Clinical Oncology, The Hebrew University, Hadassah Medical School. IIReprint requests: Abraham Amsterdam, Ph.D., Department of Hormone Research, The Weizmann Institute of Science, Rehovot 76100, Israel.
PY - 1986
Y1 - 1986
N2 - Human GCs obtained from patients undergoing an IVF procedure and treated with CC, hMG, and hCG, lack cAMP responsiveness to hCG stimulation in vitro. After 48 hours in culture, recovery of cAMP production was significantly higher in hCG-stimulated cells grown on dishes coated with ECM than in cells grown on uncoated dishes. This difference correlated with a higher degree of morphologic differentiation of cells cultured on ECM. It is concluded that ECM provides superior culture conditions for the recovery of hormone-stimulated cAMP production and the maintenance of morphologic differentiation of preovulatory GCs from stimulated cycles.
AB - Human GCs obtained from patients undergoing an IVF procedure and treated with CC, hMG, and hCG, lack cAMP responsiveness to hCG stimulation in vitro. After 48 hours in culture, recovery of cAMP production was significantly higher in hCG-stimulated cells grown on dishes coated with ECM than in cells grown on uncoated dishes. This difference correlated with a higher degree of morphologic differentiation of cells cultured on ECM. It is concluded that ECM provides superior culture conditions for the recovery of hormone-stimulated cAMP production and the maintenance of morphologic differentiation of preovulatory GCs from stimulated cycles.
UR - https://www.scopus.com/pages/publications/0022531091
U2 - 10.1016/s0015-0282(16)49596-8
DO - 10.1016/s0015-0282(16)49596-8
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AN - SCOPUS:0022531091
SN - 0015-0282
VL - 46
SP - 514
EP - 517
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 3
ER -