Crystallization of halophilic malate dehydrogenase from Halobacterium marismortui

M. Harel*, M. Shoham, F. Frolow, H. Eisenberg, M. Mevarech, A. Yonath, J. L. Sussman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Malate dehydrogenase from the extreme halophile Halobacterium marismortui crystallizes in highly concentrated phosphate solution in space group I2 with cell dimensions a = 113.8 A ̊, b = 122.8 A ̊, c = 126.7 A ̊, β = 98.1 °. The halophilic enzyme was found to be unstable at lower concentrations of phosphate. It associates with unusually large amounts of water and salt, and the combined particle volume shows a tight fit in the unit cell.

Original languageEnglish
Pages (from-to)609-610
Number of pages2
JournalJournal of Molecular Biology
Volume200
Issue number3
DOIs
StatePublished - 5 Apr 1988

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