TY - JOUR
T1 - Crystallization of a DNA tridecamer d(C-G-C-A-G-A-A-T-T-C-G-C-G)
AU - Saper, M A
AU - Eldar, H
AU - Mizuuchi, K
AU - Nickol, J
AU - Appella, E
AU - Sussman, J L
N1 - Funding Information:
We thank our colleagues David R. Davies. r\l~s Wlodawer. Maria Miller, Shula Weinst~ein. Yuan-guan Chiu, Ellen Wacht,el and Tali Haran for encouragement and suggestions throughout this work. This research supported in part bg a Cutwirth Foundat)ion grant J.L.S. and a I-nited States-Israel E:ducatjional Foundation grant t,o M.A.S.. who is a (‘harles Clore Post-doctoral Fellow.
PY - 1986/3/5
Y1 - 1986/3/5
N2 - Crystals of the DNA tridecamer d(C-G-C-A-G-A-A-T-T-C-G-C-G) have been grown by the vapor-diffusion technique with 2-methyl-2,4-pentanediol as precipitant. They are monoclinic space group C2, with a = 79.6 A, b = 43.1 A, c = 24.9 A and beta = 98.7 degrees. Previous nuclear magnetic resonance studies predicted that this tridecamer forms a duplex similar to the B DNA dodecamer, d(C-G-C-G-A-A-T-T-C-G-C-G), except for an extra adenosine residue that is stacked within the helix but remains unpaired: (formula; see text) Preliminary X-ray diffraction studies confirmed that the tridecamer is in the B DNA conformation, consistent with the nuclear magnetic resonance results.
AB - Crystals of the DNA tridecamer d(C-G-C-A-G-A-A-T-T-C-G-C-G) have been grown by the vapor-diffusion technique with 2-methyl-2,4-pentanediol as precipitant. They are monoclinic space group C2, with a = 79.6 A, b = 43.1 A, c = 24.9 A and beta = 98.7 degrees. Previous nuclear magnetic resonance studies predicted that this tridecamer forms a duplex similar to the B DNA dodecamer, d(C-G-C-G-A-A-T-T-C-G-C-G), except for an extra adenosine residue that is stacked within the helix but remains unpaired: (formula; see text) Preliminary X-ray diffraction studies confirmed that the tridecamer is in the B DNA conformation, consistent with the nuclear magnetic resonance results.
KW - Crystallography
KW - Nucleic Acid Conformation
KW - Oligodeoxyribonucleotides
KW - Polydeoxyribonucleotides
UR - http://www.scopus.com/inward/record.url?scp=0023041949&partnerID=8YFLogxK
U2 - 10.1016/0022-2836(86)90487-0
DO - 10.1016/0022-2836(86)90487-0
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C2 - 3712441
SN - 0022-2836
VL - 188
SP - 111
EP - 113
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 1
ER -