Crystallization of a DNA tridecamer d(C-G-C-A-G-A-A-T-T-C-G-C-G)

M A Saper, H Eldar, K Mizuuchi, J Nickol, E Appella, J L Sussman

Research output: Contribution to journalArticlepeer-review

Abstract

Crystals of the DNA tridecamer d(C-G-C-A-G-A-A-T-T-C-G-C-G) have been grown by the vapor-diffusion technique with 2-methyl-2,4-pentanediol as precipitant. They are monoclinic space group C2, with a = 79.6 A, b = 43.1 A, c = 24.9 A and beta = 98.7 degrees. Previous nuclear magnetic resonance studies predicted that this tridecamer forms a duplex similar to the B DNA dodecamer, d(C-G-C-G-A-A-T-T-C-G-C-G), except for an extra adenosine residue that is stacked within the helix but remains unpaired: (formula; see text) Preliminary X-ray diffraction studies confirmed that the tridecamer is in the B DNA conformation, consistent with the nuclear magnetic resonance results.

Original languageEnglish
Pages (from-to)111-3
Number of pages3
JournalJournal of Molecular Biology
Volume188
Issue number1
DOIs
StatePublished - 5 Mar 1986

Keywords

  • Crystallography
  • Nucleic Acid Conformation
  • Oligodeoxyribonucleotides
  • Polydeoxyribonucleotides

Fingerprint

Dive into the research topics of 'Crystallization of a DNA tridecamer d(C-G-C-A-G-A-A-T-T-C-G-C-G)'. Together they form a unique fingerprint.

Cite this