TY - JOUR
T1 - Crystallization and structure determination of a symmetrical 'football' complex of the mammalian mitochondrial Hsp60-Hsp10 chaperonins
AU - Nisemblat, Shahar
AU - Parnas, Avital
AU - Yaniv, Oren
AU - Azem, Abdussalam
AU - Frolow, Felix
PY - 2014/1
Y1 - 2014/1
N2 - The mitochondrial Hsp60-Hsp10 complex assists the folding of various proteins impelled by ATP hydrolysis, similar to the bacterial chaperonins GroEL and GroES. The near-Atomic structural details of the mitochondrial chaperonins are not known, despite the fact that almost two decades have passed since the structures of the bacterial chaperonins became available. Here, the crystallization procedure, diffraction experiments and structure determination by molecular replacement of the mammalian mitochondrial chaperonin HSP60 (E321K mutant) and its co-chaperonin Hsp10 are reported.
AB - The mitochondrial Hsp60-Hsp10 complex assists the folding of various proteins impelled by ATP hydrolysis, similar to the bacterial chaperonins GroEL and GroES. The near-Atomic structural details of the mitochondrial chaperonins are not known, despite the fact that almost two decades have passed since the structures of the bacterial chaperonins became available. Here, the crystallization procedure, diffraction experiments and structure determination by molecular replacement of the mammalian mitochondrial chaperonin HSP60 (E321K mutant) and its co-chaperonin Hsp10 are reported.
KW - mHsp10
KW - mHsp60
KW - mitochondrial chaperonins
UR - http://www.scopus.com/inward/record.url?scp=84901295915&partnerID=8YFLogxK
U2 - 10.1107/S2053230X1303389X
DO - 10.1107/S2053230X1303389X
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AN - SCOPUS:84901295915
SN - 1744-3091
VL - 70
SP - 116
EP - 119
JO - Acta Crystallographica Section F:Structural Biology Communications
JF - Acta Crystallographica Section F:Structural Biology Communications
IS - 1
ER -