Crystallization and preliminary X-ray analysis of a cohesin domain of the cellulosome from Clostridium thermocellum

Linda J.W. Shimon, Felix Frolow, Sima Yaron, Edward A. Bayer, Raphael Lamed, Ely Morag, Yuval Shoham

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Recombinant cohesin-2, a unique type of protein-recognition domain from the cellulosome of Clostridium thermocellum, has been crystallized by the hanging-drop vapor-diffusion method. The crystals are monoclinic, space group C2 with unit-cell dimensions a = 79.91, b = 47.86, c = 51.13 Å, β = 126.77. There is most likely to be one molecule per asymmetric unit, corresponding to a packing density of 2.16 Å3 Da-1. The crystals diffract to beyond 2.3 Å on a conventional laboratory rotating anode source.

Original languageEnglish
Pages (from-to)114-115
Number of pages2
JournalActa Crystallographica Section D: Biological Crystallography
Volume53
Issue number1
DOIs
StatePublished - 1997

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