TY - JOUR
T1 - Crystallization and initial X-ray diffraction studies of higher plant photosystem I
AU - Ben-Shem, Adam
AU - Nelson, Nathan
AU - Frolow, Felix
PY - 2003/10/1
Y1 - 2003/10/1
N2 - Complete photosystem I from a higher plant (pea; Pisum sativum) was isolated, purified and crystallized. The crystals diffract to 4 Å resolution using synchrotron radiation and belong to the monoclinic crystal system, space group P21, with unit-cell parameters a = 181.90, b = 190.24, c = 219.66 Å, β = 90.484°. Data sets from three crystals have been collected and were merged into a 4.4 Å resolution data set, taking advantage of the high isomorphicity observed for these crystals. Analysis of the self-rotation Patterson function suggests the possible presence of two PSI complexes in the asymmetric unit.
AB - Complete photosystem I from a higher plant (pea; Pisum sativum) was isolated, purified and crystallized. The crystals diffract to 4 Å resolution using synchrotron radiation and belong to the monoclinic crystal system, space group P21, with unit-cell parameters a = 181.90, b = 190.24, c = 219.66 Å, β = 90.484°. Data sets from three crystals have been collected and were merged into a 4.4 Å resolution data set, taking advantage of the high isomorphicity observed for these crystals. Analysis of the self-rotation Patterson function suggests the possible presence of two PSI complexes in the asymmetric unit.
UR - https://www.scopus.com/pages/publications/0141959896
U2 - 10.1107/S0907444903016056
DO - 10.1107/S0907444903016056
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AN - SCOPUS:0141959896
SN - 0907-4449
VL - 59
SP - 1824
EP - 1827
JO - Acta Crystallographica Section D: Structural Biology
JF - Acta Crystallographica Section D: Structural Biology
IS - 10
ER -