Coupling site I and the rotenone-sensitive ubisemiquinone in tightly coupled submitochondrial particles

A. B. Kotlyar; V. D. Sled; D. Sh Burbaev; I. A. Moroz; A. D. Vinogradov

doi:10.1016/0014-5793(90)80753-6

Coupling site I and the rotenone-sensitive ubisemiquinone in tightly coupled submitochondrial particles

A. B. Kotlyar, V. D. Sled, D. Sh Burbaev, I. A. Moroz, A. D. Vinogradov

Biochemistry Molecular Biology

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Abstract

The rotenone-sensitive g = 2.00 low temperature EPR signal attributed to ubisemiquinone is observed in submitochondrial particles during coupled electron transfer from NADH to oxygen and from succinate to NAD⁺. The signal is seen only in the presence of oligomycin added to induce the respiratory control (7-9 with NADH and 3-4 with succinate) and it disappears in the presence of uncouplers (CCCP or gramicidin D). No reduction of the iron-sulfur center N-2 in the presence of 20 mM succinate and cyanide is observed, thus suggesting that N-2 is not in equilibrium with the ubiquinone pool. A hypothesis is proposed on 7Delta;sm_H+ generation coupled with electron transfer between iron-sulfur center N-2 and the ubiquinone pool.

Original language	English
Pages (from-to)	17-20
Number of pages	4
Journal	FEBS Letters
Volume	264
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(90)80753-6
State	Published - 7 May 1990

Keywords

Electron transfer
Mitochrondria
NADH-ubiquinone reductase
Ubisemiquinone

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10.1016/0014-5793(90)80753-6

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title = "Coupling site I and the rotenone-sensitive ubisemiquinone in tightly coupled submitochondrial particles",

abstract = "The rotenone-sensitive g = 2.00 low temperature EPR signal attributed to ubisemiquinone is observed in submitochondrial particles during coupled electron transfer from NADH to oxygen and from succinate to NAD+. The signal is seen only in the presence of oligomycin added to induce the respiratory control (7-9 with NADH and 3-4 with succinate) and it disappears in the presence of uncouplers (CCCP or gramicidin D). No reduction of the iron-sulfur center N-2 in the presence of 20 mM succinate and cyanide is observed, thus suggesting that N-2 is not in equilibrium with the ubiquinone pool. A hypothesis is proposed on 7Delta;smH+ generation coupled with electron transfer between iron-sulfur center N-2 and the ubiquinone pool.",

keywords = "Electron transfer, Mitochrondria, NADH-ubiquinone reductase, Ubisemiquinone",

author = "Kotlyar, {A. B.} and Sled, {V. D.} and Burbaev, {D. Sh} and Moroz, {I. A.} and Vinogradov, {A. D.}",

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doi = "10.1016/0014-5793(90)80753-6",

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AU - Kotlyar, A. B.

AU - Sled, V. D.

AU - Burbaev, D. Sh

AU - Moroz, I. A.

AU - Vinogradov, A. D.

PY - 1990/5/7

Y1 - 1990/5/7

N2 - The rotenone-sensitive g = 2.00 low temperature EPR signal attributed to ubisemiquinone is observed in submitochondrial particles during coupled electron transfer from NADH to oxygen and from succinate to NAD+. The signal is seen only in the presence of oligomycin added to induce the respiratory control (7-9 with NADH and 3-4 with succinate) and it disappears in the presence of uncouplers (CCCP or gramicidin D). No reduction of the iron-sulfur center N-2 in the presence of 20 mM succinate and cyanide is observed, thus suggesting that N-2 is not in equilibrium with the ubiquinone pool. A hypothesis is proposed on 7Delta;smH+ generation coupled with electron transfer between iron-sulfur center N-2 and the ubiquinone pool.

AB - The rotenone-sensitive g = 2.00 low temperature EPR signal attributed to ubisemiquinone is observed in submitochondrial particles during coupled electron transfer from NADH to oxygen and from succinate to NAD+. The signal is seen only in the presence of oligomycin added to induce the respiratory control (7-9 with NADH and 3-4 with succinate) and it disappears in the presence of uncouplers (CCCP or gramicidin D). No reduction of the iron-sulfur center N-2 in the presence of 20 mM succinate and cyanide is observed, thus suggesting that N-2 is not in equilibrium with the ubiquinone pool. A hypothesis is proposed on 7Delta;smH+ generation coupled with electron transfer between iron-sulfur center N-2 and the ubiquinone pool.

KW - Electron transfer

KW - Mitochrondria

KW - NADH-ubiquinone reductase

KW - Ubisemiquinone

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Coupling site I and the rotenone-sensitive ubisemiquinone in tightly coupled submitochondrial particles

Abstract

Keywords

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