Costimulatory activity of inactivated influenza virus in response of human peripheral blood mononuclear cells to phytohemagglutinin A and anti-CD3 antibody

Avi Eisenthal; Oleg Marder; Anna Gelfend; Yehuda Skornick; Beatriz Lifschitz-Mercer

doi:10.1089/vim.1998.11.137

Costimulatory activity of inactivated influenza virus in response of human peripheral blood mononuclear cells to phytohemagglutinin A and anti-CD3 antibody

Avi Eisenthal^*, Oleg Marder, Anna Gelfend, Yehuda Skornick, Beatriz Lifschitz-Mercer

^*Corresponding author for this work

Tel Aviv Sourasky Medical Center

Research output: Contribution to journal › Article › peer-review

Abstract

We tested the effect of inactivated influenza A virus on the response of human T lymphocytes to several stimuli. Our results demonstrate that the response to both phytohemagglutinin A (PHA) and anti-CD3 was substantially enhanced by pre-exposure of peripheral blood mononuclear cells (PBMC) to heat-inactivated viral particles, whereas no effect was noted on the proliferative response of PBMC to IL-2. In addition to cell proliferation, exposure of PBMC to viral particles increased the secretion of interferon (IFN)-γ during stimulation with anti-CD3 antibody and PHA. The costimulatory effect of influenza A virus was reduced by 40% in the presence of 0.1 mM sialic acid. The possible mechanisms involved in the costimulatory effects of influenza A virus are discussed.

Original language	English
Pages (from-to)	137-145
Number of pages	9
Journal	Viral Immunology
Volume	11
Issue number	3
DOIs	https://doi.org/10.1089/vim.1998.11.137
State	Published - 1998
Externally published	Yes

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10.1089/vim.1998.11.137

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abstract = "We tested the effect of inactivated influenza A virus on the response of human T lymphocytes to several stimuli. Our results demonstrate that the response to both phytohemagglutinin A (PHA) and anti-CD3 was substantially enhanced by pre-exposure of peripheral blood mononuclear cells (PBMC) to heat-inactivated viral particles, whereas no effect was noted on the proliferative response of PBMC to IL-2. In addition to cell proliferation, exposure of PBMC to viral particles increased the secretion of interferon (IFN)-γ during stimulation with anti-CD3 antibody and PHA. The costimulatory effect of influenza A virus was reduced by 40% in the presence of 0.1 mM sialic acid. The possible mechanisms involved in the costimulatory effects of influenza A virus are discussed.",

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AU - Skornick, Yehuda

AU - Lifschitz-Mercer, Beatriz

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N2 - We tested the effect of inactivated influenza A virus on the response of human T lymphocytes to several stimuli. Our results demonstrate that the response to both phytohemagglutinin A (PHA) and anti-CD3 was substantially enhanced by pre-exposure of peripheral blood mononuclear cells (PBMC) to heat-inactivated viral particles, whereas no effect was noted on the proliferative response of PBMC to IL-2. In addition to cell proliferation, exposure of PBMC to viral particles increased the secretion of interferon (IFN)-γ during stimulation with anti-CD3 antibody and PHA. The costimulatory effect of influenza A virus was reduced by 40% in the presence of 0.1 mM sialic acid. The possible mechanisms involved in the costimulatory effects of influenza A virus are discussed.

AB - We tested the effect of inactivated influenza A virus on the response of human T lymphocytes to several stimuli. Our results demonstrate that the response to both phytohemagglutinin A (PHA) and anti-CD3 was substantially enhanced by pre-exposure of peripheral blood mononuclear cells (PBMC) to heat-inactivated viral particles, whereas no effect was noted on the proliferative response of PBMC to IL-2. In addition to cell proliferation, exposure of PBMC to viral particles increased the secretion of interferon (IFN)-γ during stimulation with anti-CD3 antibody and PHA. The costimulatory effect of influenza A virus was reduced by 40% in the presence of 0.1 mM sialic acid. The possible mechanisms involved in the costimulatory effects of influenza A virus are discussed.

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Costimulatory activity of inactivated influenza virus in response of human peripheral blood mononuclear cells to phytohemagglutinin A and anti-CD3 antibody

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