TY - JOUR
T1 - Correlation between the presence of KRAS mutation and the morphometric characteristics of colorectal carcinoma cell nuclei
AU - Greenberg, Ariel
AU - Hershkovitz, Dov
AU - Sabo, Edmond
N1 - Publisher Copyright:
© Science Printers and Publishers, Inc.
PY - 2014
Y1 - 2014
N2 - OBJECTIVE: To determine whether there is an association between KRAS status and changes in nuclear morphometric properties. STUDY DESIGN: A total of 60 cases were included in the analysis. Thirty cases carried a mutated KRAS oncogene, while 30 bore a wild-type KRAS gene. Computerized morphometry was used to measure various nuclear indices of size, shape, and texture. RESULTS: Nuclear ellipticity (p=0.02) and chromatin textural contrast (p = 0.02) were both significantly higher in the mutated KRAS group. However, chromatin contrast was the only independent predictor of KRAS status. A ROC analysis detected a best chromatin contrast cutoffpoint of 798, with a sensitivity of 89.7% and a specificity of 52%. Tumor grade was also associated with nuclear ellipticity (p=0.001). Yet, no association was found between tumor grade and KRAS status. CONCLUSION: Our results suggest that the presence of a KRAS mutation in colorectal carcinoma is associated with alterations in the nuclear morphometric propOBJECTIVE: To determine whether there is an association between KRAS status and changes in nuclear morphometric properties. STUDY DESIGN: A total of 60 cases were included in the analysis. Thirty cases carried a mutated KRAS oncogene, while 30 bore a wild-type KRAS gene. Computerized morphometry was used to measure various nuclear indices of size, shape, and texture. RESULTS: Nuclear ellipticity (p=0.02) and chromatin textural contrast (p=0.02) were both significantly higher in the mutated KRAS group. However, chromatin contrast was the only independent predictor of KRAS status. A ROC analysis detected a best chromatin contrast cutoffpoint of 798, with a sensitivity of 89.7% and a specificity of 52%. Tumor grade was also associated with nuclear ellipticity (p=0.001). Yet, no association was found between tumor grade and KRAS status. CONCLUSION: Our results suggest that the presence of a KRAS mutation in colorectal carcinoma is associated with alterations in the nuclear morphometric properties of contrast and ellipticity.
AB - OBJECTIVE: To determine whether there is an association between KRAS status and changes in nuclear morphometric properties. STUDY DESIGN: A total of 60 cases were included in the analysis. Thirty cases carried a mutated KRAS oncogene, while 30 bore a wild-type KRAS gene. Computerized morphometry was used to measure various nuclear indices of size, shape, and texture. RESULTS: Nuclear ellipticity (p=0.02) and chromatin textural contrast (p = 0.02) were both significantly higher in the mutated KRAS group. However, chromatin contrast was the only independent predictor of KRAS status. A ROC analysis detected a best chromatin contrast cutoffpoint of 798, with a sensitivity of 89.7% and a specificity of 52%. Tumor grade was also associated with nuclear ellipticity (p=0.001). Yet, no association was found between tumor grade and KRAS status. CONCLUSION: Our results suggest that the presence of a KRAS mutation in colorectal carcinoma is associated with alterations in the nuclear morphometric propOBJECTIVE: To determine whether there is an association between KRAS status and changes in nuclear morphometric properties. STUDY DESIGN: A total of 60 cases were included in the analysis. Thirty cases carried a mutated KRAS oncogene, while 30 bore a wild-type KRAS gene. Computerized morphometry was used to measure various nuclear indices of size, shape, and texture. RESULTS: Nuclear ellipticity (p=0.02) and chromatin textural contrast (p=0.02) were both significantly higher in the mutated KRAS group. However, chromatin contrast was the only independent predictor of KRAS status. A ROC analysis detected a best chromatin contrast cutoffpoint of 798, with a sensitivity of 89.7% and a specificity of 52%. Tumor grade was also associated with nuclear ellipticity (p=0.001). Yet, no association was found between tumor grade and KRAS status. CONCLUSION: Our results suggest that the presence of a KRAS mutation in colorectal carcinoma is associated with alterations in the nuclear morphometric properties of contrast and ellipticity.
KW - Colon cancer
KW - Colon carcinoma
KW - Colorectal carcinoma
KW - KRAS
KW - KRAS oncogene
UR - http://www.scopus.com/inward/record.url?scp=84903757950&partnerID=8YFLogxK
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C2 - 24902368
AN - SCOPUS:84903757950
SN - 0884-6812
VL - 36
SP - 23
EP - 31
JO - Analytical and Quantitative Cytology and Histology
JF - Analytical and Quantitative Cytology and Histology
IS - 1
ER -
