Correlated Selection on Amino Acid Deletion and Replacement in Mammalian Protein Sequences

Yichen Zheng, Dan Graur, Ricardo B.R. Azevedo

Research output: Contribution to journalArticlepeer-review

Abstract

A low ratio of nonsynonymous and synonymous substitution rates (dN/dS) at a codon is an indicator of functional constraint caused by purifying selection. Intuitively, the functional constraint would also be expected to prevent such a codon from being deleted. However, to the best of our knowledge, the correlation between the rates of deletion and substitution has never actually been estimated. Here, we use 8595 protein-coding region sequences from nine mammalian species to examine the relationship between deletion rate and dN/dS. We find significant positive correlations at the levels of both sites and genes. We compared our data against controls consisting of simulated coding sequences evolving along identical phylogenetic trees, where deletions occur independently of substitutions. A much weaker correlation was found in the corresponding simulated sequences, probably caused by alignment errors. In the real data, the correlations cannot be explained by alignment errors. Separate investigations on nonsynonymous (dN) and synonymous (dS) substitution rates indicate that the correlation is most likely due to a similarity in patterns of selection rather than in mutation rates.

Original languageEnglish
Pages (from-to)365-378
Number of pages14
JournalJournal of Molecular Evolution
Volume86
Issue number6
DOIs
StatePublished - 1 Jul 2018
Externally publishedYes

Keywords

  • Codon deletion
  • Indifferent DNA
  • Mammals
  • Protein-coding genes
  • Purifying selection
  • dN/dS

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