@article{e03a6460d77f44728f1382ed094b1175,
title = "Core-binding specificity of bacteriophage integrases",
abstract = "The site-specific recombination systems of bacteriophages λ and HK022 share the same mechanism and their integrase proteins show strong homology. Nevertheless the integrase protein of each phage can only catalyze recombination between its own att sites. Previous work has shown that the specificity determinants in the att sites are located within the sequences that bind the integrase to the core of att. DNA fragments that carry attL and attR sites of each phage were challenged with each of the two integrases and the DNA-protein complexes were examined by the gel-retardation technique. The results show that each integrase can form higher-order DNA-protein complexes only with its cognate att sites, suggesting that differences in the mode of binding to the core are responsible for the specificity difference between the two integrases.",
keywords = "Bacteriophage HK022, Bacteriophage λ, DNA-protein interaction, Integrase, Site-specific recombination",
author = "P. Gottfried and E. Yagil and M. Kolot",
note = "Funding Information: Acknowledgments Thanks to Dr. Hagg{\aa}rd-Ljungquist for sending plasmid pEE2003, and to Gilad Mass for helping with the Figures. This work was supported by the Israel Science Foundation founded by The Academy of Sciences and Humanities, and by the Ela Kodesz Institute for Research on Cancer Development and Prevention.",
year = "2000",
doi = "10.1007/s004380051209",
language = "אנגלית",
volume = "263",
pages = "619--624",
journal = "Molecular and General Genetics",
issn = "0026-8925",
publisher = "Springer Verlag",
number = "4",
}