Copii collar defines the boundary between er and er exit site and does not coat cargo containers

Olga Shomron, Inbar Nevo-Yassaf, Tamar Aviad, Yakey Yaffe, Eitan Erez Zahavi, Anna Dukhovny, Eran Perlson, Ilya Brodsky, Adva Yeheskel, Metsada Pasmanik-Chor, Anna Mironov, Galina V. Beznoussenko, Alexander A. Mironov, Ella H. Sklan, George H. Patterson, Yoji Yonemura, Mara Sannai, Christoph Kaether*, Koret Hirschberg*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


COPII and COPI mediate the formation of membrane vesicles translocating in opposite directions within the secretory pathway. Live-cell and electron microscopy revealed a novel mode of function for COPII during cargo export from the ER. COPII is recruited to membranes defining the boundary between the ER and ER exit sites, facilitating selective cargo concentration. Using direct observation of living cells, we monitored cargo selection processes, accumulation, and fission of COPII-free ERES membranes. CRISPR/Cas12a tagging, the RUSH system, and pharmaceutical and genetic perturbations of ER-Golgi transport demonstrated that the COPII coat remains bound to the ER–ERES boundary during protein export. Manipulation of the cargobinding domain in COPII Sec24B prohibits cargo accumulation in ERES. These findings suggest a role for COPII in selecting and concentrating exported cargo rather than coating Golgi-bound carriers. These findings transform our understanding of coat proteins’ role in ER-to-Golgi transport.

Original languageEnglish
Article numbere201907224
JournalJournal of Cell Biology
Issue number6
StatePublished - 2021


FundersFunder number
Nihal Altan Bonnet
National Institutes of Health
National Institute of Biomedical Imaging and BioengineeringZIAEB000071
Israel Science Foundation1063/16


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