Controlled microtubules transport on patterned non-fouling surfaces

R. C. Lipscomb, J. Clemmens, Y. Hanein, M. R. Holl, V. Vogel, B. D. Ratner, D. D. Denton, K. F. Böhringer

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Described is a lithographic technique used to realize high resolution tracks of the motor protein kinesin inside flow cells. The process consists of patterns of plasma polymerized PEG-like non-fouling coating on a fouling glass substrate. When in contact with a coated surface, proteins adhere exclusively to the fouling areas, thus forming kinesin tracks suited for microtubule guidance. Parallel transport along smooth kinesin track was observed for over 250 μm. A method has been developed to seal the substrates with molded silicon flow channels in order to allow convenient control of reagent introduction and immobilization processes. Complete kinesin and BSA immobilization protocols, comprised of initial rinse, protein introduction, immobilization and final rinse have been successfully demonstrated.

Original languageEnglish
Title of host publication2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings
EditorsDavid Beebe, Andre Dittmar
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages21-26
Number of pages6
ISBN (Electronic)0780374800, 9780780374805
DOIs
StatePublished - 2002
Externally publishedYes
Event2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Madison, United States
Duration: 2 May 20024 May 2002

Publication series

Name2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings

Conference

Conference2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology
Country/TerritoryUnited States
CityMadison
Period2/05/024/05/02

Keywords

  • Protein patterns
  • kinesin
  • microfluidics
  • microtubules
  • non-fouling coatings
  • plasma deposition

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