@inproceedings{93f161cee5444044a9050549793f2c70,
title = "Controlled microtubules transport on patterned non-fouling surfaces",
abstract = "Described is a lithographic technique used to realize high resolution tracks of the motor protein kinesin inside flow cells. The process consists of patterns of plasma polymerized PEG-like non-fouling coating on a fouling glass substrate. When in contact with a coated surface, proteins adhere exclusively to the fouling areas, thus forming kinesin tracks suited for microtubule guidance. Parallel transport along smooth kinesin track was observed for over 250 μm. A method has been developed to seal the substrates with molded silicon flow channels in order to allow convenient control of reagent introduction and immobilization processes. Complete kinesin and BSA immobilization protocols, comprised of initial rinse, protein introduction, immobilization and final rinse have been successfully demonstrated.",
keywords = "Protein patterns, kinesin, microfluidics, microtubules, non-fouling coatings, plasma deposition",
author = "Lipscomb, {R. C.} and J. Clemmens and Y. Hanein and Holl, {M. R.} and V. Vogel and Ratner, {B. D.} and Denton, {D. D.} and B{\"o}hringer, {K. F.}",
note = "Publisher Copyright: {\textcopyright} 2002 IEEE.; 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology ; Conference date: 02-05-2002 Through 04-05-2002",
year = "2002",
doi = "10.1109/MMB.2002.1002257",
language = "אנגלית",
series = "2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
pages = "21--26",
editor = "David Beebe and Andre Dittmar",
booktitle = "2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings",
address = "ארצות הברית",
}