Abstract
Two alternative enzymatic routes to continuous biological acetaldehyde production from ethanol by immobilized yeast were compared: ADH of Candida utilis and AOX of Candida boidinii. The ADH route, performed by immobilized cells in the presence of tris buffer continuously trapping the acetaldehyde formed, was found superior to the AOX route. It exhibited significantly higher operational stability in both batchwise or continuous operation in fluidized bed reactor. The ADH system exhibited a stable acetaldehyde production level for 5 d, followed by gradual decrease in activity, which could be readily fully regenerated in situ by flushing with growth medium. Efficient continuous product trapping by tris buffer was essential to maintain continuous operation.
Original language | English |
---|---|
Pages (from-to) | 387-399 |
Number of pages | 13 |
Journal | Applied Biochemistry and Biotechnology |
Volume | 39-40 |
Issue number | 1 |
DOIs | |
State | Published - Sep 1993 |
Keywords
- Acetaldehyde
- Candida utilis
- alcohol dehydrogenase
- alcohol oxidase
- immobilized yeast
- product recovery