Continuous production of acetaldehyde by immobilized yeast with in situ product trapping - Comparison of alcohol dehydrogenase and alcohol oxidase routes

Yael Shachar-Nishri, Amihay Freeman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Two alternative enzymatic routes to continuous biological acetaldehyde production from ethanol by immobilized yeast were compared: ADH of Candida utilis and AOX of Candida boidinii. The ADH route, performed by immobilized cells in the presence of tris buffer continuously trapping the acetaldehyde formed, was found superior to the AOX route. It exhibited significantly higher operational stability in both batchwise or continuous operation in fluidized bed reactor. The ADH system exhibited a stable acetaldehyde production level for 5 d, followed by gradual decrease in activity, which could be readily fully regenerated in situ by flushing with growth medium. Efficient continuous product trapping by tris buffer was essential to maintain continuous operation.

Original languageEnglish
Pages (from-to)387-399
Number of pages13
JournalApplied Biochemistry and Biotechnology
Volume39-40
Issue number1
DOIs
StatePublished - Sep 1993

Keywords

  • Acetaldehyde
  • Candida utilis
  • alcohol dehydrogenase
  • alcohol oxidase
  • immobilized yeast
  • product recovery

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