TY - JOUR
T1 - Consistent levels of A-to-I RNA editing across individuals in coding sequences and non-conserved Alu repeats
AU - Greenberger, Shoshana
AU - Levanon, Erez Y.
AU - Paz-Yaacov, Nurit
AU - Barzilai, Aviv
AU - Safran, Michal
AU - Osenberg, Sivan
AU - Amariglio, Ninette
AU - Rechavi, Gideon
AU - Eisenberg, Eli
N1 - Funding Information:
We thank Ofra Maydan Sherf for performing the sequenome analysis. S.G was supported by Talpiot Medical Leadership Program; Sheba Medical Center, Israel. E.Y.L was supported by the Israel Science Foundation (Legacy Heritage Science Initiative). We thank the Flight Attendant Medical Research Institute (FAMRI) for their support. The work of E.E. was supported by the Israel Science Foundation [grant number 365/06] and the Israel Ministry for Science and Technology (Scientific Infrastructure Program).
PY - 2010/10/28
Y1 - 2010/10/28
N2 - Background: Adenosine to inosine (A-to-I) RNA-editing is an essential post-transcriptional mechanism that occurs in numerous sites in the human transcriptome, mainly within Alu repeats. It has been shown to have consistent levels of editing across individuals in a few targets in the human brain and altered in several human pathologies. However, the variability across human individuals of editing levels in other tissues has not been studied so far.Results: Here, we analyzed 32 skin samples, looking at A-to-I editing level in three genes within coding sequences and in the Alu repeats of six different genes. We observed highly consistent editing levels across different individuals as well as across tissues, not only in coding targets but, surprisingly, also in the non evolutionary conserved Alu repeats.Conclusions: Our findings suggest that A-to-I RNA-editing of Alu elements is a tightly regulated process and, as such, might have been recruited in the course of primate evolution for post-transcriptional regulatory mechanisms.
AB - Background: Adenosine to inosine (A-to-I) RNA-editing is an essential post-transcriptional mechanism that occurs in numerous sites in the human transcriptome, mainly within Alu repeats. It has been shown to have consistent levels of editing across individuals in a few targets in the human brain and altered in several human pathologies. However, the variability across human individuals of editing levels in other tissues has not been studied so far.Results: Here, we analyzed 32 skin samples, looking at A-to-I editing level in three genes within coding sequences and in the Alu repeats of six different genes. We observed highly consistent editing levels across different individuals as well as across tissues, not only in coding targets but, surprisingly, also in the non evolutionary conserved Alu repeats.Conclusions: Our findings suggest that A-to-I RNA-editing of Alu elements is a tightly regulated process and, as such, might have been recruited in the course of primate evolution for post-transcriptional regulatory mechanisms.
UR - http://www.scopus.com/inward/record.url?scp=79952073022&partnerID=8YFLogxK
U2 - 10.1186/1471-2164-11-608
DO - 10.1186/1471-2164-11-608
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 21029430
AN - SCOPUS:79952073022
SN - 1471-2164
VL - 11
JO - BMC Genomics
JF - BMC Genomics
IS - 1
M1 - 608
ER -