Conserved quartets near 5′ intron junctions in primate nuclear pre-mRNA

Ruth Nussinov*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Analysis of a 1000 nucleotide span around 664 primate 5′ exon/intron junctions revealed frequent recurrences of G-rich runs downstream of the 5′ splice sites. In particular, AGGG, GGGA, GGGG, GGGT and TGGG are frequent at this site. Some C-rich quartets are frequent upstream of the 5′ splice site. Similar behaviour of these G- and C-rich quartets is indicated for the 587 rodent introns and for a combined eukaryotic file containing 1688 introns. (A)GGG(A) is also frequent in the introns 60 nucleotides upstream of the 3′ splice site, and (A)CCC(A) is frequently found in the exons downstream of the 3′ site. The same consistent behaviour of the 3′ splice sites is obtained as for the 5′ sites, for the primates, rodents and combined eukaryotic file. These results suggest that in addition to the well-conserved 5′ and 3′ splice sequences, exon as well as intron sequences may play a role in nuclear pre-mRNA splicing.

Original languageEnglish
Pages (from-to)73-84
Number of pages12
JournalJournal of Theoretical Biology
Volume133
Issue number1
DOIs
StatePublished - 7 Jul 1988

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