Concanavalin a receptors on the surface membrane of lymphocytes from patients with acute leukemia

Hannah Ben-Bassat; Eliahou Anor; Shmuel Penchas; Zipora Shlomai; Miron Prokocimer; Reuven Or; Aaron Polliack

doi:10.1016/0145-2126(84)90024-9

Concanavalin a receptors on the surface membrane of lymphocytes from patients with acute leukemia

Hannah Ben-Bassat^*, Eliahou Anor, Shmuel Penchas, Zipora Shlomai, Miron Prokocimer, Reuven Or, Aaron Polliack

^*Corresponding author for this work

Hadassah University Medical Centre

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Peripheral blood mononuclear cells (PBM) isolated from 23 patients with acute lymphoblastic leukemia (ALL) and 24 with acute non-lymphoblastic leukemia (ANLL) were studied for binding and mobility of Concanavalin A (Con A) receptors, using fluorescent Con A (F-Con-A). The cap forming ability of PBM from all patients was 18.7 (± 9.3%) and 18.9 (± 9.9%) for ANLL patients at the time of diagnosis or during relapse. During clinical complete remission the cap forming ability of the PBM did not change significantly. No correlation was observed between the percentage of blasts present in the peripheral blood at the time of examination and the extent of cap formation, for both types of leukemia. The pattern of F-Con-A binding to PBM in ANLL patients was different compared to that seen in ALL. In ANLL, the fluorescent stain was concentrated in a round body on the cell ("button form") after binding to the membrane, while the rest of the cell showed almost no fluorescence. The present results indicate that PBM cells from patients with acute leukemia are characterized by a high degree of Con-A receptor mobility.

Original language	English
Pages (from-to)	745-752
Number of pages	8
Journal	Leukemia Research
Volume	8
Issue number	4
DOIs	https://doi.org/10.1016/0145-2126(84)90024-9
State	Published - 1984
Externally published	Yes

Funding

Funders	Funder number
Alexander and Sophia S. Goodman
Leonard Wolfson Research Fund of the Hebrew University of Jerusalem

Keywords

Acute leukemia
Concanavalin A
capping
lymphocytes
surface membrane

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0145-2126(84)90024-9

Cite this

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title = "Concanavalin a receptors on the surface membrane of lymphocytes from patients with acute leukemia",

abstract = "Peripheral blood mononuclear cells (PBM) isolated from 23 patients with acute lymphoblastic leukemia (ALL) and 24 with acute non-lymphoblastic leukemia (ANLL) were studied for binding and mobility of Concanavalin A (Con A) receptors, using fluorescent Con A (F-Con-A). The cap forming ability of PBM from all patients was 18.7 (± 9.3%) and 18.9 (± 9.9%) for ANLL patients at the time of diagnosis or during relapse. During clinical complete remission the cap forming ability of the PBM did not change significantly. No correlation was observed between the percentage of blasts present in the peripheral blood at the time of examination and the extent of cap formation, for both types of leukemia. The pattern of F-Con-A binding to PBM in ANLL patients was different compared to that seen in ALL. In ANLL, the fluorescent stain was concentrated in a round body on the cell ({"}button form{"}) after binding to the membrane, while the rest of the cell showed almost no fluorescence. The present results indicate that PBM cells from patients with acute leukemia are characterized by a high degree of Con-A receptor mobility.",

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author = "Hannah Ben-Bassat and Eliahou Anor and Shmuel Penchas and Zipora Shlomai and Miron Prokocimer and Reuven Or and Aaron Polliack",

note = "Funding Information: This work was supported in part by the Leonard Wolfson Research Fund of the Hebrew University of Jerusalem (H.B.) and from funds donated by Alexander and Sophia S. Goodman, via PEF Endowment Funds, Inc., New York, U.S.A. (A.P.) Abbreviations: ALL, acute lymphoblastic leukemia; ANLL, acute non-lymphoblastic leukemia; PBM, peri. pheral blood mononuclear cells; R, relapse; CR, complete remission; ICR, incomplete remission. Correspondence to: H. Ben-Bassat, Laboratory of Experimental Surgery, Hadassah University Hospital, Jerusalem, Israel.",

year = "1984",

doi = "10.1016/0145-2126(84)90024-9",

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T1 - Concanavalin a receptors on the surface membrane of lymphocytes from patients with acute leukemia

AU - Ben-Bassat, Hannah

AU - Anor, Eliahou

AU - Penchas, Shmuel

AU - Shlomai, Zipora

AU - Prokocimer, Miron

AU - Or, Reuven

AU - Polliack, Aaron

N1 - Funding Information: This work was supported in part by the Leonard Wolfson Research Fund of the Hebrew University of Jerusalem (H.B.) and from funds donated by Alexander and Sophia S. Goodman, via PEF Endowment Funds, Inc., New York, U.S.A. (A.P.) Abbreviations: ALL, acute lymphoblastic leukemia; ANLL, acute non-lymphoblastic leukemia; PBM, peri. pheral blood mononuclear cells; R, relapse; CR, complete remission; ICR, incomplete remission. Correspondence to: H. Ben-Bassat, Laboratory of Experimental Surgery, Hadassah University Hospital, Jerusalem, Israel.

PY - 1984

Y1 - 1984

N2 - Peripheral blood mononuclear cells (PBM) isolated from 23 patients with acute lymphoblastic leukemia (ALL) and 24 with acute non-lymphoblastic leukemia (ANLL) were studied for binding and mobility of Concanavalin A (Con A) receptors, using fluorescent Con A (F-Con-A). The cap forming ability of PBM from all patients was 18.7 (± 9.3%) and 18.9 (± 9.9%) for ANLL patients at the time of diagnosis or during relapse. During clinical complete remission the cap forming ability of the PBM did not change significantly. No correlation was observed between the percentage of blasts present in the peripheral blood at the time of examination and the extent of cap formation, for both types of leukemia. The pattern of F-Con-A binding to PBM in ANLL patients was different compared to that seen in ALL. In ANLL, the fluorescent stain was concentrated in a round body on the cell ("button form") after binding to the membrane, while the rest of the cell showed almost no fluorescence. The present results indicate that PBM cells from patients with acute leukemia are characterized by a high degree of Con-A receptor mobility.

AB - Peripheral blood mononuclear cells (PBM) isolated from 23 patients with acute lymphoblastic leukemia (ALL) and 24 with acute non-lymphoblastic leukemia (ANLL) were studied for binding and mobility of Concanavalin A (Con A) receptors, using fluorescent Con A (F-Con-A). The cap forming ability of PBM from all patients was 18.7 (± 9.3%) and 18.9 (± 9.9%) for ANLL patients at the time of diagnosis or during relapse. During clinical complete remission the cap forming ability of the PBM did not change significantly. No correlation was observed between the percentage of blasts present in the peripheral blood at the time of examination and the extent of cap formation, for both types of leukemia. The pattern of F-Con-A binding to PBM in ANLL patients was different compared to that seen in ALL. In ANLL, the fluorescent stain was concentrated in a round body on the cell ("button form") after binding to the membrane, while the rest of the cell showed almost no fluorescence. The present results indicate that PBM cells from patients with acute leukemia are characterized by a high degree of Con-A receptor mobility.

KW - Acute leukemia

KW - Concanavalin A

KW - capping

KW - lymphocytes

KW - surface membrane

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JO - Leukemia Research

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ER -

Concanavalin a receptors on the surface membrane of lymphocytes from patients with acute leukemia

Abstract

Funding

Keywords

UN SDGs

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